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A method for increasing the mannan content of yeast space mutagenesis strains

A technology of mannan and yeast, which is applied in the field of increasing the mannan content of yeast space mutagenesis strains, can solve the problems of affecting the yield and affecting the bioactive cell wall synthesis of yeast cells, so as to improve the utilization rate and overcome the single and good fermentation method. The effect of applying the foreground

Active Publication Date: 2019-03-08
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Medium components can affect the biological activity of yeast cells and the synthesis of cell walls, thereby affecting their yield

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, a kind of method that improves the mannan content of yeast space mutagenesis strain

[0038] 1. Preparation of fermentation medium

[0039] The preparation method of the fermentation medium: mix 4.51g sucrose (Beijing Chemical Reagent), 4.76g corn steep liquor (Shandong Province Zouping Fenglin Feed Co., Ltd.), 1.20g glycerin (Beijing Chemical Reagent) and water, and dilute to 100ml to obtain the fermentation medium, so that the concentration of sucrose in the fermentation medium is 4.51g / 100ml, the concentration of corn steep liquor in the fermentation medium is 4.76g / 100ml, and the concentration of glycerol in the fermentation medium is 1.20g / 100ml .

[0040] 2. Fermentation and cultivation of Saccharomyces cerevisiae mutant strain FFLM2.0016

[0041] 1. Strain activation treatment

[0042] Use the fermentation medium of step 1 to activate the Saccharomyces cerevisiae mutant strain FFLM2.0016. The specific steps are as follows: Pick 2-3 loops of the S...

Embodiment 2

[0048] Embodiment 2, a kind of method for improving the mannan content of yeast space mutagenesis strain

[0049] 1. Preparation of fermentation medium

[0050] The preparation method of the fermentation medium: mix 4.51g sucrose, 4.76g corn steep liquor, 1.20g glycerin and water, and set the volume to 100ml to obtain the fermentation medium, so that the concentration of sucrose in the fermentation medium is 4.51g / 100ml 1. The concentration of corn steep liquor in the fermentation medium is 4.76g / 100ml, and the concentration of glycerol in the fermentation medium is 1.20g / 100ml.

[0051] 2. Fermentation and cultivation of Saccharomyces cerevisiae mutant strain FFLM2.0016

[0052] 1. Strain activation treatment

[0053] Use the fermentation medium of step 1 to activate the Saccharomyces cerevisiae mutant strain FFLM2.0016. The specific steps are as follows: Pick 2-3 loops of the Saccharomyces cerevisiae mutant strain FFLM2.0016 strain preserved on the slope and inoculate it i...

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Abstract

The invention discloses a method for increasing content of mannan in space mutation yeast strains. According to the method, fermentation culture is performed on saccharomyces cerevisiae with a culture medium comprising a carbon source, a nitrogen source and an enzyme activator, and the mannan is obtained. Tests prove that the content and the biomass of the mannan obtained with the method are 321.89 plus / minus 1.94 mg / 100 ml and 3846.22 plus / minus 9.28 mg / 100 ml respectively and are 75.69% and 91.24% higher than those of basic culture medium YEPD (yeast extract peptone dextrose) respectively. The method overcomes defects that existing fermentation means are single, extracted polysaccharide content is lower and the like, all adopted equipment can meet requirements of industrial production, the cost is low, the economic benefit is high, and the method has the very good application prospect.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for increasing the mannan content of yeast space mutagenesis strains. Background technique [0002] The yeast cell wall accounts for 20-25% of the dry weight of the cell, and mannan exists in the outer layer of the yeast cell wall, accounting for about 40% of the dry weight of the cell wall, endowing the cell with biological activity and controlling the pore size of the cell wall. Mannan has a variety of immunological functions, such as regulating the balance of intestinal flora, enhancing immunity, binding or adsorbing mycotoxins, anti-oxidation, etc., and has broad application prospects. [0003] Space-mutated yeast strains are usually obtained from Saccharomyces cerevisiae through space-borne mutagenesis carried by satellites, which have the characteristics of shortened growth delay period, prolonged logarithmic growth period, accelerated growth rate,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04
Inventor 刘红芝王强刘丽石爱民胡晖巩阿娜李亚楠林伟静段玉权
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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