Fritillaria thun-bergli cholinesterase activity-inhibition extract product, medical purpose of its composition and preparation method thereof
A technology of extracting and Fritillaria, applied in the field of medicine, can solve the problems of high price and small quantity
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Embodiment 1
[0100] Take 20 g of the Uighur medicine Fritillaria, ultrasonically extract it with 100 mL methanol for 15 min, and then ultrasonically extract it with 100 mL water for 15 min. After the extract is concentrated, it is separated with a silica gel column. First use chloroform-methanol mixed solvent (chloroform-methanol, 100:4, volume ratio) to elute for 15 times the column volume, and then use chloroform-methanol mixed solvent (chloroform-methanol, 100:12, volume ratio) to elute , eluted for 25 times the column volume, and the eluate eluted with a chloroform-methanol mixed solvent (chloroform-methanol, 100:12, volume ratio) was recovered as the organic solvent and dried in vacuum to obtain the active extract. After testing, its butyrylcholinesterase inhibitory activity is IC 50 =82.0 μg / mL.
Embodiment 2
[0102] Take 20 g of the Uyghur medicine Fritillaria, ultrasonically extract it with 100 mL ethanol for 15 min, concentrate the extract, and separate it with a silica gel column. -methanol, 100:4, volume ratio) elution, elution 15 times the column volume, followed by elution with chloroform-methanol mixed solvent (chloroform-methanol, 100:12, volume ratio) elution, elution 25 times the column volume, with The eluate eluted with chloroform-methanol mixed solvent (chloroform-methanol, 100:12, volume ratio) is recovered as the organic solvent and freeze-dried to obtain the active extract. After testing, its butyrylcholinesterase inhibitory activity is IC 50 =88.0 μg / mL.
Embodiment 3
[0104] Take 20 g of the Uyghur medicine Fritillaria, ultrasonically extract it with 100 mL of petroleum ether for 15 min, concentrate the extract, and then separate it with a silica gel column. Chloroform-methanol, 100:4, volume ratio) elution, elution 15 times the column volume, followed by chloroform-methanol mixed solvent (chloroform-methanol, 100:12, volume ratio) elution, elution 25 times the column volume, The eluate eluted with chloroform-methanol mixed solvent (chloroform-methanol, 100:12, volume ratio) is the active extract after recovering the organic solvent and drying by rotary evaporation. After testing, its butyrylcholinesterase inhibitory activity is IC 50 =76.0 μg / mL.
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