Method for detecting serum biochemical indices or sub-health improvement effect of immune cells
A technology of immune cells and biochemical indicators, applied in the field of biochemistry, can solve problems such as no effect evaluation indicators
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Embodiment 1
[0106] Example 1 Detection of the effect of immune cells on improving sub-health
[0107] (1) Preparation of NK cell preparations
[0108] 1. Ficoll separation method: separate and obtain peripheral blood mononuclear cells (PBMC), press (1-2)×10 6 Add LONZA's X-VIVO15 serum-free medium at the density of cell / ml, and add IL-2, IL-15, IL-21, and OKT-3 factors.
[0109] 2. Add X-VIVOX15 serum-free medium every 3 days, and according to the volume after rehydration, add IL-2, IL-15, IL-21, OKT-3 factors to cultivate 12-16 to obtain the required NK cells, collected in NK cell preparations were obtained by injecting physiological saline.
[0110] (2) Detection scheme
[0111] Fasting serum UA, TC, TG, HDL-C, LDL-C biochemical indicators were detected before, 1 month and 3 months after NK cell reinfusion. The detection indicators are as follows:
[0112] Table 1 NK cell curative effect detection index
[0113] Table of contents
before return
January after reinf...
Embodiment 2
[0131] Example 2 Detection example
[0132] Two patients with chronic insomnia and similar physical conditions were selected. Five biochemical indicators were detected respectively. Patient A was reinfused with the same volume of normal saline, and patient B was given autologous NK cells (3×10 8 ~9×10 8 ) + normal saline reinfusion, 5 biochemical indicators were tested one month later, and recent sleep quality problems were recorded; 5 biochemical indicators were tested 3 months later, and recent sleep quality problems were recorded. The result is as follows:
[0133] Table 2 Detection results of biochemical indicators in the first experiment
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[0136] Result analysis:
[0137] The five biochemical indexes of patient A did not change significantly before reinfusion, after 1 month, and after 3 months, among which TG and LDL-C were within the normal range, and UA, TC, and HDL-C were on the high or high side. Low; no improvement in night sleep duration...
Embodiment 3
[0143] Example 3 Detection example
[0144] Randomly select 10 sub-healthy people aged 30-60 (with varying degrees of insomnia, endocrine disorders, memory loss, inattention, etc.), and carry out the same experimental process as in Example 1 respectively.
[0145] 2-5 of the 5 biochemical indicators in the population with significant improvement returned to normal values, and the variation range exceeded 5%;
[0146] In the slightly improved population, at least one of the five biochemical indicators returned to the normal range after 1 month, and the variation range exceeded 5%; or at least 2 of the 5 biochemical indicators returned to the normal range after 3 months, and the variation range exceeded 5%.
[0147] The above results show that the method provided by the present invention can objectively detect the improvement effect of immune cells on the serum biochemical indicators of sub-healthy people, and can objectively detect the improvement effect of immune cells on sub-...
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