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Immunoaffinity column for simultaneous analysis of four mycotoxins (DON, ZEA, T-2, HT-2) and preparation method thereof

A technology of HT-2 and mycotoxins, applied in the direction of analyzing materials, material separation, measuring devices, etc., to achieve the effect of improving use efficiency, advanced technology and unique method

Inactive Publication Date: 2015-09-23
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no efficient trichothecene mycotoxin multi-target (DON, ZEA, T-2, HT-2) immunoaffinity column at the same time to achieve simultaneous purification of several mycotoxins, which greatly saves time and cost

Method used

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  • Immunoaffinity column for simultaneous analysis of four mycotoxins (DON, ZEA, T-2, HT-2) and preparation method thereof

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Embodiment Construction

[0008] Specific examples are given by the legends. The legend shows a process flow chart for the preparation of an immunoaffinity column for the simultaneous analysis of four mycotoxins (DON, ZEA, T-2, HT-2), which includes antibody preparation and purification 1, matrix preparation 2, Ligand coupling 3, column packing 4.

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Abstract

An immunoaffinity column for simultaneous analysis of four mycotoxins (DON, ZEA, T-2, HT-2) and a preparation method thereof belong to the preparation techniques. The method includes the preparation and purification of antibodies, the preparation of a matrix, ligand coupling and column packing. The invention has novel design, advanced technology, unique method and wide purpose, not only solves the problems of matrix interference, low specificity and high cost in the traditional enrichment process of trichothecene mycotoxins, but also achieves simultaneous purification of multiple targets. The immunoaffinity column has the advantages of strong specificity, great improvement of the use efficiency, easy operation, low production cost, and strong practicality and generalization performance.

Description

technical field [0001] The invention relates to a preparation method of a mycotoxin (DON, ZEA, T-2, HT-2) four-in-one immunoaffinity column, which belongs to the preparation technology. Background technique [0002] Trichothecene mycotoxins are a large family of chemically related mycotoxins in Fusarium toxins, which are very harmful to the health of humans and livestock. They are widely found in various food crops and processed products, and are often found in humid environments In moldy grains stored in China. There are four types that are often detected from contaminated grain and feed: T-2 toxin, HT-2 toxin, deoxynivalenol DON and zearalenone ZEA. For the determination and analysis of trichothecene mycotoxins in food products, the main methods are chromatography, including gas chromatography-mass spectrometry (GC-MS), liquid chromatography (LC), liquid chromatography / mass spectrometry (LC-MS) and liquid chromatography / tandem mass spectrometry (LC-MS / MS), etc. These ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N30/60
CPCG01N33/543G01N30/60
Inventor 丁轲张家宁韩涛陈湘宁
Owner BEIJING UNIV OF AGRI
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