Mussel protein antihypertensive peptide

A technology for antihypertensive peptides and proteins, applied in the field of bioengineering, can solve problems such as preparing antihypertensive peptides, and achieve the effects of improving utilization, scientific and reasonable process, and high activity

Active Publication Date: 2015-09-30
安徽盛美诺生物技术有限公司
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  • Mussel protein antihypertensive peptide

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Embodiment

[0024] A rapid preparation method of mussel protein antihypertensive peptide, the preparation process is as follows: mussel meat "tissue crushing" enzymatic hydrolysis "ultrafiltration" agarose magnetic microspheres immobilized ACE adsorption "RP-HPLC purification" antihypertensive peptide.

[0025] ) Preparation and activation of agarose magnetic microspheres: According to the weight volume ratio of 1.5g: 30 mL, add agarose to water, boil together until the agarose is completely dissolved, add 1 ml Fe 3 o 4 Ferrofluid, fully mixed on a vortex mixer; drop the mixed liquid into the organic phase at 80°C (Span-80: liquid paraffin=1g:25mL) under stirring, and react at 80°C for 20 min; the organic phase Cool to 40°C and mechanically stir at 250 rpm for 15 min to shape the microspheres, and separate them in a magnetic field. The prepared microspheres were washed three times with petroleum ether and deionized water to remove the residual organic phase to obtain agarose magnetic M...

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Abstract

The invention discloses a mussel protein antihypertensive peptide with an antihypertensive activity as well as a preparation method and an application thereof. The amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp (VSWPCRW), and the molecular weight measured by ESI-MS is 935.07 Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp (VSWPCRW) is obtained through shelling and meat taking, enzymolysis, ultrafiltration, agarose magnetic microsphere immobilized ACE adsorption and reversed-phase high-performance liquid chromatography (RP-HPLC) purification. The active peptide prepared by the preparation method disclosed by the invention is remarkable in angiotensin converting enzyme (ACE) inhibitory activity, and capable of being used for hypertension treatment-related medicines.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a mussel protein antihypertensive peptide. Background technique [0002] Angiotensin-converting enzyme (ACE), also known as peptidyl-carboxypeptidase or kininase II, has a molecular weight of 150,000 Da and belongs to the membrane-bound enzyme of vascular endothelial cells. ACE can hydrolyze the decapeptide angiotensin I into the octapeptide angiotensin II, which promotes further constriction of blood vessels, leading to an increase in blood pressure. At the same time, ACE can also promote the secretion of aldosterone by acting on the adrenal cortex. Therefore, ACE is an important component of renin-angiotensin-aldosterone (RAS). In addition, ACE can also catalyze the hydrolysis of bradykinin, which has antihypertensive effect, and make it inactive. Therefore, the purpose of lowering or controlling blood pressure can be achieved by finding a suitable substan...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61P9/12
Inventor 王斌迟长凤赵玉勤孙坤来
Owner 安徽盛美诺生物技术有限公司
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