Adriamycin-resistant U87 cell model and application thereof
A cell model and doxorubicin-resistant technology, applied in animal cells, tumor/cancer cells, vertebrate cells, etc., can solve the problems of poor effect and easy drug resistance, and achieve good drug resistance stability
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Embodiment 1
[0024] 1. Experimental materials
[0025] U87 cells were purchased from Chinese Academy of Sciences.
[0026] Doxorubicin (DOX) was purchased from KGI Biotech (Nanjing, China).
[0027] Doxorubicin working solution was prepared with cell culture medium (SH30243.01B+10% FBS, purchased from HyClone, USA).
[0028] 2. Experimental method
[0029] 1) U87 cells were cultured with SH30243.01B+10% FBS+100 U / ml penicillin+0.1 mg / ml streptomycin.
[0030] 2) Start the intervention with DOX at an initial concentration of 1ng / ml, and change to the drug-free medium (SH30243.01B+10%FBS+100U / ml penicillin+0.1mg / ml streptomycin) after 24 hours of DOX administration.
[0031] 3) After the cells grow stably, the shape is plump, there are no floating dead cells, and the passage density is 30%, the cells can grow to 90% in 3 to 5 days, then start normal passage (P) and increase the concentration of DOX.
[0032] Mother cell U87 was intervened with 1ng / ml DOX from the fifth passage (P5) to P1...
Embodiment 2
[0044]In the process of establishing the human astroglioma cell line U87R-DOX, the inventor obtained another doxorubicin-resistant U87 cell model U87R-DOX' in parallel experiments, its IC50 was 685ng / ml, and the drug resistance coefficient (48 hours) was 20.15, the drug resistance coefficient (1 month) was 14.32, and the drug resistance coefficient (6 months) was 12.80. Drug resistance and resistance stability were significantly worse than human astroglioma cell line U87R-DOX.
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