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Screening method of main active drug components in compound traditional Chinese medicine

A drug and compound technology, which is applied in the field of screening and evaluation of main-effect drug components in traditional Chinese medicine compounds, can solve the problems of poor portability, too strong subjectivity, and no indication of main-effect drug components in traditional Chinese medicine compounds.

Inactive Publication Date: 2018-05-04
INST OF BASIC RES & CLINICAL MEDICINE CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is too dependent on experimental design and artificially selected drug targets, which is too subjective and poor in portability, and the results do not give the main active drug ingredients in the traditional Chinese medicine compound, which cannot provide support for further development of combination drugs

Method used

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  • Screening method of main active drug components in compound traditional Chinese medicine
  • Screening method of main active drug components in compound traditional Chinese medicine
  • Screening method of main active drug components in compound traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. Experimental materials

[0066] FVB / NJ mice: purchased from Shanghai Experimental Animal Research Center;

[0067] Acute promyelocytic leukemia (APL) mouse model: constructed according to literature methods;

[0068] Compound Huangdai Tablets: purchased from Guangzhou Baiji Xinte Pharmacy;

[0069] Realgar, Danshen, Qingdai: purchased from Caizhilin Pharmacy in Guangzhou;

[0070] Affymetrix GeneChip Mouse Genome4302.0Array: purchased from Shanghai Biochip Co., Ltd.;

[0071] R: open source computing platform, downloaded from R official website;

[0072] Limma analysis package: open source analysis package, downloaded from the Bioconductor website;

[0073] Protein interaction data: downloaded from the BioGRID database.

[0074] 2. Experimental method:

[0075] (1) Fifteen 6-8 week FVB / NJ mice were injected with cells expressing PML-RAR to establish a mouse model of acute promyelocytic leukemia (APL);

[0076] (2) Divide APL mice into groups of 3 each, one gro...

Embodiment 2

[0091] 1. Experimental materials

[0092] SCID mice: purchased from Guangdong Medical Experimental Animal Center;

[0093] Acute promyelocytic leukemia (APL) mouse model: constructed according to literature methods;

[0094] Compound Huangdai Tablets: purchased from Guangzhou Baiji Xinte Pharmacy;

[0095] Realgar, Danshen, Qingdai: purchased from Caizhilin Pharmacy in Guangzhou;

[0096] Illumina MouseRef-8v2.0expression beadchip: purchased from Shanghai Biochip Co., Ltd.;

[0097] R: open source computing platform, downloaded from R official website;

[0098] Limma analysis package: open source analysis package, downloaded from the Bioconductor website;

[0099] Protein interaction data: download from BioGRID database;

[0100] 2. Experimental method

[0101] (1) 15 6-8 week SCID mice were injected with cells expressing PML-RAR to establish a mouse model of acute promyelocytic leukemia (APL);

[0102](2) Divide APL mice into groups of 3 each, one group is used as APL ...

Embodiment 3

[0117] 1. Experimental materials

[0118] BABL / c mice: purchased from Guangdong Medical Experimental Animal Center;

[0119] Acute promyelocytic leukemia (APL) mouse model: constructed according to literature methods;

[0120] Compound Huangdai Tablets: purchased from Guangzhou Baiji Xinte Pharmacy;

[0121] Realgar, Danshen, Qingdai: purchased from Caizhilin Pharmacy in Guangzhou;

[0122] Agilent-014868Whole Mouse Genome Microarray4x44K G4122F: purchased from Shanghai Biochip Co., Ltd.;

[0123] R: open source computing platform, downloaded from R official website;

[0124] Limma analysis package: open source analysis package, downloaded from the Bioconductor website;

[0125] Protein interaction data: download from BioGRID database;

[0126] 2. Experimental method

[0127] (1) Fifteen BABL / c mice at 6-8 weeks were injected with cells expressing PML-RAR to establish a mouse model of acute promyelocytic leukemia (APL);

[0128] (2) Divide APL mice into groups of 3 each...

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Abstract

The invention relates to the field of medicines and particularly discloses a screening method of main-effect medicine components in a traditional Chinese medicine compound. The screening method includes the following steps: (1) grouping experimental animals and delivering medicines to the experimental animals; (2) finding a target point gene; (3) calculating influence value; (4) calculating the ratio value of medicine effects and determining a main effect component; and (5) determining the main-effect medicine components in the traditional Chinese medicine compound according to the ratio value of the medicine effect of single medicine. The method not only is closely combined with a modern high-throughput technology being mature and reliable, but also conforms to the theories in traditional Chinese medicine and modern medicine and pharmacology, so that researching on the effective substances in the traditional Chinese medicine compound is more scientific and modern, thereby providing an important basis for further researching a combined medicine.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a method for screening and evaluating main active drug components in a compound traditional Chinese medicine. Background technique [0002] The screening and evaluation of main active drugs in compound Chinese medicines is an important part of the modernization research of Chinese medicines, and it is the basis for further research and development of combined drugs based on compound Chinese medicines. The chemical composition of traditional Chinese medicine is extremely complex, and a single medicinal material itself is a complex mixture, and a traditional Chinese medicine compound prescription composed of several or even dozens of medicinal materials is the basic form of traditional Chinese medicine. [0003] Therefore, exploring the material basis of traditional Chinese medicine effects is not only a very arduous task but also a requirement of modern medical treatmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 何冰梁超何小鹃吕诚姜淼牛旭艳刘彪郭保生刘进党蕾李德芳张戈吕爱平
Owner INST OF BASIC RES & CLINICAL MEDICINE CHINA ACAD OF CHINESE MEDICAL SCI
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