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Large-scale artificial cultivation method for phellinus igniarius sporocarp

A technology of artificial cultivation and Phellinus chinensis, which is applied in the fields of cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of restricting the development of Phellinus phellibilis industry, difficulty in large-scale production, and difficulty in artificial domestication and cultivation of Phellinus chinensis, and achieve standardized quality and technical parameter control, reducing the need for wood resources, and standardizing the effects of artificial cultivation models

Active Publication Date: 2022-05-10
内蒙古科学技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the increasing demand for Phellinus Phellinus in the market has made it impossible to eliminate the predatory exploitation of Phellinus Phellinus, and due to the particularity and complexity of its physiological ecology and the constraints of external conditions, the fruiting bodies formed in nature are very rare, wild and natural The resources of Phellinus Phellinus are scarce and facing depletion. In addition, it is difficult to domesticate and cultivate Phellinus Phellinus artificially, and it is difficult to form a stable source of pharmaceutical industry products, which greatly limits the development of Phellinus Phellinus industry.
[0004] At present, there are two main methods of artificial cultivation, one is the cultivation of segmented trees such as mulberry and poplar, which requires a large amount of wood resources and is difficult to produce on a large scale, and the other is planting with bag materials. The cultivation medium has the problems of insufficient nutrition, small fruit body development, and susceptibility to miscellaneous bacteria, and the use of solid-state medium has the problems of uneven dispersion of bacteria and long growth cycle
At the same time, the use of liquid medium for artificial cultivation has the advantages of convenient inoculation, short cultivation period, low pollution rate, controllable and consistent inoculum quantity, and rapid colonization. However, there are still liquid medium formulas that are single and costly. Technical problems limit its large-scale application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for large-scale artificial cultivation of Phellinus radix fruiting bodies, comprising the following steps:

[0032] (1) Preparation of solid medium

[0033] Weigh 40 parts of cottonseed hulls, 30 parts of mulberry wood chips, 10 parts of bran, 3 parts of glucose, 2 parts of sucrose, 1 part of hydroxyapatite, 3 parts of potassium dihydrogen phosphate, 1 part of calcium sulfate, and 6 parts of sodium alginate. Evenly, control the water content to 70% to obtain a solid medium;

[0034] (2) Preparation of liquid culture medium

[0035] Using water as a solvent, each liter of culture medium includes the following raw materials by weight: corn flour 10g, potassium dihydrogen phosphate 5g, magnesium sulfate 3g, glucose 15g, vitamin B 0.005g, peptone 5g, magnesium acetate tetrahydrate 0.5g; Mix with water evenly to obtain a liquid culture medium;

[0036] (3) Production of bacteria sticks

[0037] Select a hollow cylindrical plastic pipe fitting, add the liquid med...

Embodiment 2

[0049] A method for large-scale artificial cultivation of Phellinus radix fruiting bodies, comprising the following steps:

[0050] (1) Preparation of solid medium

[0051] Weigh 20 parts of cottonseed hulls, 60 parts of mulberry wood chips, 10 parts of bran, 3 parts of glucose, 2 parts of sucrose, 1 part of hydroxyapatite, 2 parts of potassium dihydrogen phosphate, 1 part of calcium sulfate, and 4 parts of sodium alginate. Evenly, control the water content to 65% to obtain a solid medium;

[0052](2) Preparation of liquid culture medium

[0053] Using water as a solvent, each liter of culture medium includes the following raw materials by weight: corn flour 15g, potassium dihydrogen phosphate 5g, magnesium sulfate 1g, glucose 12g, vitamin B 0.004g, peptone 8g, magnesium acetate tetrahydrate 1g; Mix evenly with water to obtain a liquid medium;

[0054] (3) Production of bacteria sticks

[0055] Select a hollow cylindrical plastic pipe fitting, add the liquid medium to the ...

Embodiment 3

[0066] A method for large-scale artificial cultivation of Phellinus radix fruiting bodies, comprising the following steps:

[0067] (1) Preparation of solid medium

[0068] Weigh 30 parts of cottonseed hulls, 50 parts of mulberry wood chips, 6 parts of bran, 2 parts of glucose, 3 parts of sucrose, 1 part of hydroxyapatite, 5 parts of potassium dihydrogen phosphate, 3 parts of calcium sulfate, and 6 parts of sodium alginate. Evenly, control the water content to 70% to obtain a solid medium;

[0069] (2) Preparation of liquid culture medium

[0070] Using water as a solvent, each liter of culture medium includes the following raw materials by weight: corn flour 15g, potassium dihydrogen phosphate 3g, magnesium sulfate 3g, glucose 10g, vitamin B 0.005g, peptone 8g, magnesium acetate tetrahydrate 0.5g; Mix with water evenly to obtain a liquid culture medium;

[0071] (3) Production of bacteria sticks

[0072] Select a hollow cylindrical plastic pipe fitting, add the liquid med...

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PUM

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Abstract

According to the method, the hollow cylindrical plastic pipe fitting is selected, the liquid culture medium is added into the hollow part, and the two ends of the plastic pipe fitting are filled with the solid culture medium to prepare the mushroom sticks, so that the phellinus igniarius sporocarp is cultured, and the defects of wood waste and long growth period of the solid culture medium and insufficient nutrition, easy infection and high cost of the liquid culture medium are avoided; the culture medium integrates the advantages of a solid culture medium and a liquid culture medium, the mushroom stick is easy to manufacture and low in cost, the probability of scratching a mushroom bag can be reduced, pollution can be avoided, and a good foundation can be provided for the standardized cultivation process of phellinus igniarius. According to the technological process, standardized quality and technical parameter management and control can be achieved, and a mechanical, standardized, industrialized, large-scale and standardized artificial cultivation mode of phellinus igniarius can be achieved.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of Phellinus radix, in particular to a method for large-scale artificial cultivation of Phellinus radix fruiting bodies. Background technique [0002] Phellinus is a medicinal fungus belonging to the Basidiomycotina Phyllomycetes, also known as Phellinus baumannii, because its fruiting body has multiple functions such as anti-tumor and anti-oxidation, it has become a research hotspot at home and abroad. It is an internationally recognized fungus with the highest biological anti-cancer efficiency. [0003] In recent years, the increasing demand for Phellinus Phellinus in the market has made it impossible to eliminate the predatory exploitation of Phellinus Phellinus, and due to the particularity and complexity of its physiological ecology and the constraints of external conditions, the fruiting bodies formed in nature are very rare, wild and natural The resources of Phellinus Phelli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 张秀娟侯亚光随洋
Owner 内蒙古科学技术研究院
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