A fermentation medium of Azospirillum brasiliensis with high production of ammonia and indole acetic acid and its application

Abstract

The present invention relates to Azospirillum brasiliensis R fermentation medium with high yield of ammonia and indoleacetic acid and its application, which can effectively solve the problem of high yield of ammonia and high yield of indoleacetic acid on the same medium. The method is calculated by the following weight percentage : Yeast powder 0.25~1%, soybean meal powder 0.5~2%, NaCL 0.1~1.0%, CuSO4 0.001~0.005%, tryptophan 0.01%~0.15% and the balance is made of water, first tryptophan with 0.01 M NaOH solution was dissolved, and 0.22 μm filter membrane was used to filter and sterilize to obtain a tryptophan solution with a mass concentration of 10.2115%. Then, mix yeast powder, soybean meal powder, NaCl, CuSO4 and water evenly, sterilize at 121°C for 30 minutes, and cool to room temperature. Then add tryptophan solution and mix well, and adjust the pH to 7.0-9.0 with hydrochloric acid or NaOH; the invention provides a fermentation medium and culture conditions that are simple, easy to operate, low in production cost, high in ammonia and indole acetic acid output, and easy to industrialized production. Energy saving and environmental protection, huge economic and social benefits.

Description

technical field [0001] The invention relates to microorganisms, in particular to a high-yield ammonia and indole acetic acid Azospirillum brasiliensis R fermentation medium and its application. Background technique [0002] In recent years, nitrogen-fixing bacteria, as an excellent biological nitrogen-fixing resource, have been extensively studied at home and abroad. Studies have shown that nitrogen-fixing bacteria can reduce molecular nitrogen to ammonia. Most of the nitrogen fixed by the autogenous nitrogen-fixing system is used to form the bacteria itself. During the nitrogen-fixing process, ammonia will not accumulate in the cells, and little ammonia will be secreted into the cell. Outside the bacteria. Released only after the bacteria die. The symbiotic nitrogen fixation system can directly secrete most of the assimilated ammonia for the host to absorb and utilize. The combined nitrogen fixation system is a type between autogenesis and symbiosis, but regardless of the...

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Problems solved by technology

[0005] For above-mentioned situation, in order to overcome the defect of prior art, the object of the present invention is exactly to provide a kind of Azospirillum brasiliensis R fermentation culture medium and its application of high yield ammonia and indoleacetic acid, can effectively solve the problem of high ammonia yield on the same culture medium. The problem of high yield of indole acetic acid