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Resistance identification method for sweet potato scurf

A technology for identification of resistance and black mole disease, which is applied in the field of agricultural plant protection, can solve the problems of non-resistant black mole disease and evaluation of resistance to black mole disease of sweet potato varieties, and achieve scientific identification methods, shorten value-added time, and avoid errors Effect

Inactive Publication Date: 2015-10-28
JIANGSU XUZHOU SWEET POTATO RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the sweet potato varieties promoted in production are of good quality and high commodity value, but they are not resistant to black mole disease, and there is no report on the identification method of sweet potato black mole disease resistance, and it is impossible to make a conclusion on the resistance of sweet potato varieties to black mole disease. scientific evaluation

Method used

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  • Resistance identification method for sweet potato scurf
  • Resistance identification method for sweet potato scurf
  • Resistance identification method for sweet potato scurf

Examples

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Embodiment

[0027] (1) Isolation and preparation of pathogenic bacteria: collect black mole disease-infected potato pieces from the field, and then bring them back to the laboratory to isolate the black mole pathogen according to the tissue separation method, and check the potato pieces back in accordance with Koch’s law; two weeks before sweet potato planting, At 28°C, activate and proliferate black nevus pathogenic bacteria on Chape's medium plate with peptone as nitrogen source; wash the cultivated black nevus pathogenic bacteria plate with sterile water to obtain spore suspension, and dilute the concentration of the spore suspension to 1.0×10 6 pcs / ml or so;

[0028] (2) Participating varieties and preparation of potato seedlings: Select Shengli 100, Jinshan 57, Xinzhonghua, Lizixiang, Shangshu 19, Shangshu 7, Xushu 22, Xushu 18, Yanshu 25 , Jixu 23 and other 10 sweet potato varieties tested. The tested sweet potato varieties were seeded normally in the seedbed in the first ten days...

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Abstract

The invention discloses a resistance identification method for sweet potato scurf, and belongs to resistance identification methods for crop diseases and pests. 30 healthy and strong sweet potato seedlings of a to-be-identified sweet potato are shorn from a seedling bed with a high seedling shearing method at three times in the middle of June, and 10 seedlings are shorn each time. When the sweet potato seedlings are planted, the roots are dipped with bacterial suspension having the concentration being 1.0*10<6> bacteria / ml, and a conventional mode is adopted for planting and daily field management. After being planted for 120 days, the sweet potato seedlings are harvested and stored in a sweet potato storage house, and during the storage, the temperature is controlled to be about 12-15 DEG C and the relative humidity is controlled to be 80%-90%. In 150 days, sweet potato blocks are taken out, cleaned and dried in air, the disease grades are classified according to the surface area of scurf scabs in the sweet potato blocks, the disease index is calculated, grading is carried out by the disease index, and the anti-infection property of a variety is evaluated. The identification method is scientific, effective and simple to operate; the test cycle covers the whole infection and extension cycle of the sweet potato scurf during field growth and winter storage of sweet potatoes; and an identification result can comprehensively reflect the resistance level of the sweet potato variety on the scurf.

Description

technical field [0001] The invention relates to a method for identifying resistance to crop diseases and insect pests, in particular to a method for identifying resistance to sweet potato black mole disease, belonging to the field of agricultural plant protection. Background technique [0002] Sweetpotato mole disease (sweetpotato mole disease) is caused by the half-knowledge fungus subphylum Sweetpotato Alternaria (Monilochaetes infuscans Ell. et Halst. Ex Harter), and it occurs in every sweet potato producing area in my country. The disease generally only infects the potato skin, does not penetrate into the potato meat, has no significant impact on the yield, and has not attracted people's attention for a long time. Potato pieces are easy to lose moisture after being infected, and tend to shrink during storage, which affects quality and edible value. In addition, with the rise of the fresh sweet potato industry, the damage caused by black mole has become an important fact...

Claims

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Application Information

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IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 赵永强谢逸萍杨冬静孙厚俊张成玲徐振
Owner JIANGSU XUZHOU SWEET POTATO RES CENT
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