Resistance identification method for sweet potato scurf
A technology for identification of resistance and black mole disease, which is applied in the field of agricultural plant protection, can solve the problems of non-resistant black mole disease and evaluation of resistance to black mole disease of sweet potato varieties, and achieve scientific identification methods, shorten value-added time, and avoid errors Effect
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[0027] (1) Isolation and preparation of pathogenic bacteria: collect black mole disease-infected potato pieces from the field, and then bring them back to the laboratory to isolate the black mole pathogen according to the tissue separation method, and check the potato pieces back in accordance with Koch’s law; two weeks before sweet potato planting, At 28°C, activate and proliferate black nevus pathogenic bacteria on Chape's medium plate with peptone as nitrogen source; wash the cultivated black nevus pathogenic bacteria plate with sterile water to obtain spore suspension, and dilute the concentration of the spore suspension to 1.0×10 6 pcs / ml or so;
[0028] (2) Participating varieties and preparation of potato seedlings: Select Shengli 100, Jinshan 57, Xinzhonghua, Lizixiang, Shangshu 19, Shangshu 7, Xushu 22, Xushu 18, Yanshu 25 , Jixu 23 and other 10 sweet potato varieties tested. The tested sweet potato varieties were seeded normally in the seedbed in the first ten days...
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