Identification method for adaptability of plant to low-phosphorus environment

An identification method and an adaptive technology, applied in the field of ecological governance, can solve the problems of restricting plant growth and development in karst areas, the limitation of inorganic phosphorus supply, etc., and achieve the effect of fewer steps, reliable data and simple calculation

Active Publication Date: 2015-11-04
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

At the same time, during the karstification process of the bedrock, hydrogen ions are also consumed to form a large amount of calcium ions and bicarbonate ions, which is bound to transform the overlying soil so that the overlying soil presents a high pH, ​​high calcium and In the environment of high bicarbonate, in the alkaline lime soil in the karst area, due to the high adsorption and insolubility of phosphorus, the form of phosphorus in the soil cannot be transformed into inorganic phosphorus (HPO) required for plant growth. 4 2- or H 2 PO 4 - ) for direct absorption, the supply of inorganic phosphorus is severely limited, restricting the growth and development of plants in karst areas

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  • Identification method for adaptability of plant to low-phosphorus environment
  • Identification method for adaptability of plant to low-phosphorus environment

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example

[0040] Example of the present invention: it comprises the following steps:

[0041] First, hydroponically culture the investigated plants to a suitable size, select seedlings of the same size, and starve them for 48 hours in a culture solution without adding inorganic phosphorus with a certain number of seedlings as a unit;

[0042] Second, the plant seedlings under investigation after starvation culture were placed in an additional 0.2 mmol / L CaSO 4 Dihydrogen phosphate ion absorption experiments were carried out in the absorption solution containing dihydrogen phosphate ions.

[0043] Third, according to the above-mentioned ion absorption experiments, the Michaelis constants of the dihydrogen phosphate ion absorption of the investigated plants were respectively obtained K m and the minimum concentration, that is, the external nutrient concentration when the net absorption rate of dihydrogen phosphate ion by plant roots is equal to zero C min .

[0044] Fourth, measure t...

Embodiment 1

[0053] Example 1 Adaptability of mulberry and mulberry to the low phosphorus environment of lime soil in karst area

[0054] The following is an example of measuring the adaptability of mulberry and mulberry to the low-phosphorus environment of lime soil in the karst area to show its specific implementation steps and implementation effects.

[0055] The mulberry tree and mulberry tree were used as experimental materials. Take mature mulberry and mulberry seeds, sow part of them in the low-phosphorus environment to be tested (lime soil in karst area) and let them grow for 4 months, then take the rhizosphere soil of mulberry and mulberry trees to analyze the available phosphorus content in the soil. Part of the experiments were carried out on the absorption of dihydrogen phosphate ions.

[0056] The dihydrogen phosphate ion absorption experiment is:

[0057] Seeds were sown in the soil of the greenhouse to grow seedlings. After two true leaves grew, they were transplanted ...

Embodiment 2

[0064] Example 2 The adaptability of Zhuge vegetable and rapeseed to the low phosphorus environment of lime soil in karst area to be tested

[0065] Using the method of the present invention to obtain the Michaelis constant absorbed by the dihydrogen phosphate ion of Zhuge Cai and Rapeseed K m , minimum concentration C min and available phosphorus content in the rhizosphere environment C E ,. Table 2 shows the relative absorption rate of inorganic phosphorus in the tested karst lime soil of Zhuge vegetable and rapeseed.

[0066] Table 2 The relative absorption rate of inorganic phosphorus absorption in karst lime soil of Zhuge vegetable and rapeseed

[0067]

[0068] It can be seen from Table 2 that both Zhugecai and rape have strong adaptability to the low phosphorus environment of the karst lime soil under investigation. The rapeseed used here is Brassica napus. Zhuge Cai is a plant suitable for karst growth, and its strong adaptability to low phosphorus may be ...

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Abstract

The invention discloses an identification method for adaptability of a plant to the low-phosphorus environment. The method is characterized in that hydroponics is conducted on the plant to be surveyed; then, after starving culture is conducted on the plant for 48 h in a culture solution without inorganic phosphorus, a dihydrogen phosphate ion absorption experiment is conducted, according to the Michaelis constant and the minimum concentration, obtained in the dihydrogen phosphate ion absorption experiment, of dihydrogen phosphate ion absorption of the plant to be surveyed, the available phosphorus content of the riphzosphere environment of the plant to be surveyed grown in the environment to be tested, and the relative absorption rate of dihydrogen phosphate ions of the plant to be surveyed is further calculated according to the Michaelis constant and the minimum concentration of the dihydrogen phosphate ion absorption and the available phosphorus content of the riphzosphere environment; finally, the adaptability of the plant to the low-phosphorus environment is evaluated according to the obtained relative absorption rate of the plant to be surveyed. The method is few in steps, simple in calculation, reliable in data and capable of rapidly identifying the environmental adaptability of different plants to the same low-phosphorus environment and provides a technological support for sorting plants suitable for growing in karst and terricolous floating plants.

Description

technical field [0001] The invention relates to a method for identifying the adaptability of plants to a low phosphorus environment, belonging to the field of ecological management. Background technique [0002] Inorganic phosphorus (P) in soil is one of the most important limiting factors affecting plant growth. Numerous studies have shown that phosphorus deficiency affects the photosystem of plants (Photosystem ) process, resulting in the closure of stomata, the decrease of net photosynthetic rate, and the change of chlorophyll content and chlorophyll fluorescence parameters. [0003] China's karst (karst) distribution area is about 1.3 million Km 2 , of which the rocky desertification area accounts for about 28.7%. "South rock (rocky desertification) and northern sand (desertification)" have become two major ecological environmental problems that restrict the sustainable development of the western region. The bedrock in karst habitats is mostly carbonate rocks, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 吴沿友张开艳杭红涛饶森陆叶姚凯谢腾祥刘莹
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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