A rapid breeding method for sweet potato varieties resistant to black spot
An anti-black spot and black spot disease technology, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of heavy workload, long breeding period, high cost, etc., and achieve the reduction of the number of single plants and the breeding method Simple, less work effect
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Embodiment 1
[0028] 1. Breeding process
[0029] (1) Hybrid combination configuration
[0030] Lushu 7, which is highly resistant to black spot and has high yield potential, was used as the female parent, and Jishu 15, which was resistant to black spot, was used as the male parent to sexually cross, and 3500 F1 hybrid seeds were obtained.
[0031] (2) Single plant screening of F 1 generation sweet potato
[0032] Sow the obtained 3,500 hybrid seeds in the vermiculite powder in the greenhouse, strengthen management after emergence, topping to promote branch growth, and select 3,000 healthy and healthy plants; each individual plant selects 4 growths that are basically the same, 4-5 Branches with internode length (about 15cm); 3 of them were subjected to inoculation induction of black spot conidia (Method A) (induction of black spot conidia refers to: implanting water-washed spores In sand, use a concentration of 1*10 4 individuals / ml black spot conidia suspension, watered and cultured at ...
Embodiment 2
[0046] 1. Breeding process
[0047] (1) Hybrid combination configuration
[0048] Ji 09184 with high resistance to black spot disease was used as the female parent, and Jishu 16 with good resistance to black spot disease was used as the male parent, and 1500 F1 hybrid seeds were obtained by sexual crossing.
[0049] (2) Single plant screening of F 1 generation sweet potato
[0050]Sow the obtained 1500 hybrid seeds in the vermiculite powder in the greenhouse, strengthen management after emergence, topping to promote branch growth, and select 1200 healthy and healthy plants. Each individual plant selects 4 growths that are basically the same, 4-5 Branches with an internode length (about 15cm). Among them, 3 branches were implanted in fine sand for inoculation induction of black spot conidia (Method A), and the highly susceptible variety Xushu 18 was used as a control. After 6 days, it was screened according to Table 1 of Example 1 to obtain a disease grade of 0. 9 individual...
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