Application of Arabidopsis atacs2 Gene in Drought Resistance and Early Maturity of Cotton

A technology of Arabidopsis and cotton, applied in the field of genetic engineering

Active Publication Date: 2018-04-17
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is not yet known whether the expression activity of ACS2 is related to the maturation and senescence process of cotton, and whether water absorption and utilization affect the related maturation and senescence process

Method used

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  • Application of Arabidopsis atacs2 Gene in Drought Resistance and Early Maturity of Cotton
  • Application of Arabidopsis atacs2 Gene in Drought Resistance and Early Maturity of Cotton
  • Application of Arabidopsis atacs2 Gene in Drought Resistance and Early Maturity of Cotton

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The model plant Arabidopsis ( Arabidopsis thaliana L.) provides good genetic material for revealing various physiological response mechanisms of plants. The research on the expression and regulation of related genes in Arabidopsis and the mechanism of plant response to water shortage and drought, water absorption and utilization can provide good clues and references for the research of other large-genome crops, so this example introduces the simulation of model plants. The research status of AtACS2 gene in A. thaliana.

[0038] Arabidopsis AtACS2 (the number in the Arabidopsis genome is At1g01480) encodes an ACC synthetase isoenzyme in Arabidopsis, with a CDS length of 1491bp and a protein encoding 496 amino acids.

[0039] Experimental materials: Arabidopsis thaliana AtACS2 loss-of-function mutant is a T-DNA insertion mutant, purchased from the American Arabidopsis Resource Center (ABRC, Ohio State University).

[0040] Experimental purpose: To analyze the drought r...

Embodiment 2

[0052] The GhACS2 gene of upland cotton is coded as, GenBank: ABA00145.1 or DQ174260.1; the CDS length of the gene is 2296bp, encoding a protein of 663 amino acids, and the encoded protein belongs to the ACC synthetase isozyme.

[0053] Upland cotton GhACS2 and Arabidopsis AtACS2 are homologous genes. Studies have shown that the homology of the encoded protein sequence is 63.17% (data from the National Center for Biotechnology Information in the United States, such as figure 1 shown), which is one of the highest values ​​among the known ACS protein sequence comparisons. Therefore, on the basis of the study on the function of the Arabidopsis AtACS2 gene in Example 1, the inventors believe that by enhancing the expression of the Arabidopsis AtACS2 gene and transfecting cotton, it is possible to endow cotton with agronomic traits such as drought tolerance, cotton fiber prematurity and not premature aging , On this basis, the inventor has further done a verification test, which i...

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Abstract

The application belongs to the technical field of genetic engineering, and specifically relates to the application of Arabidopsis thaliana ACS2 gene (AtACS2) gene in drought resistance and early maturity of cotton. The gene number is At1g01480, its CDS length is 1491bp, and it encodes 496 amino acids. The encoded protein belongs to an isoenzyme of ACC synthetase; after the enhanced expression of the gene, the transfected cotton can obtain drought-resistant, precocious and non-premature transgenic cotton new strain or variety. Planting experiments show that when encountering extreme water shortage and drought, compared with non-transgenic cotton, transgenic cotton has a well-developed root system, significantly increased lateral roots, and shortened internodes of cotton plants, which is conducive to water acquisition, transportation and utilization, and enhances the ability of cotton to tolerate drought At the same time, the transgenic cotton blooms and opens bolls in advance, without premature senescence, cotton fiber yield and quality are slightly improved, and has the excellent trait of "early maturity and no premature senility", which has good popularization and application value.

Description

technical field [0001] The application belongs to the technical field of genetic engineering, and specifically relates to the application of Arabidopsis thaliana ACS2 gene (AtACS2) gene in drought resistance and early maturity of cotton. Background technique [0002] Drought and water shortage endanger crop production. Water shortage factors mainly include: first, the environment is dry and water-scarce, and plants have no water available due to factors such as uneven distribution of rainwater and seasonal water shortages; Water absorption and transportation capacity is low, water transportation and utilization obstacles in cells, and high temperature causes excessive water evaporation in plants, etc. Taking cotton production in central and northern my country as an example: during the vegetative growth period of cotton, it often encounters stresses such as high temperature in summer, drought and water shortage, while the cotton harvest period often encounters unfavorable m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/52C12N15/82A01H5/00A01H6/60
Inventor 江静韩栓张华梁勋张治恩
Owner HENAN UNIVERSITY
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