Protection effect of cyanidin-3-glucoside for UVB (ultraviolet B)-induced HaCaT cell injury

A technology of glucoside and cyanidin, which is applied in the direction of medical preparations containing active ingredients, cosmetic preparations, skin care preparations, etc., to protect DNA from damage, improve survival rate, prevent and treat skin light damage effect

Active Publication Date: 2015-11-11
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although previous studies have shown that C3G has powerful anti-inflammatory and anti-oxidative properties, there is no relevant research on whether C3G has the effect of reducing photodamage on human epidermal cells and its safety. and reports

Method used

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  • Protection effect of cyanidin-3-glucoside for UVB (ultraviolet B)-induced HaCaT cell injury
  • Protection effect of cyanidin-3-glucoside for UVB (ultraviolet B)-induced HaCaT cell injury
  • Protection effect of cyanidin-3-glucoside for UVB (ultraviolet B)-induced HaCaT cell injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Study of cyanidin-3-glucoside interfering with ROS / COX-2 pathway in HaCaT cells damaged by UVB

[0052] 1. Experimental method

[0053] 1. HaCaT cell culture

[0054] (1) Cell Recovery

[0055] 1) Take out the cryopreservation tube containing HaCaT cells from the -80°C refrigerator, put it into warm water at 37°C immediately, and shake it from time to time to make it evenly heated and melt as soon as possible;

[0056] 2) When the cryopreservation solution is completely dissolved, take out the cryopreservation tube, suck out the cell suspension in a sterile table, inject it into 9ml of cell culture medium, blow it evenly, and centrifuge at 1000rpm×5min;

[0057] 3) Discard the supernatant, add 6ml medium, pipette the discrete cells, transfer to 10mm Petri dish, place in 5% CO 2 , Cultured in a 37°C incubator.

[0058] (2) Cell culture

[0059] 1) Cells were grown in DMEM medium containing 10% FBS and placed in 5% CO 2 , Cultivate in a 37°C incubator; c...

Embodiment 2C3

[0172] The effect of embodiment 2C3G on the apoptosis of HaCaT cells after UVB irradiation

[0173] 1. Apoptosis detection——AnnexinV-FITC / PI double staining flow cytometry detection

[0174] The principle of AnnexinV-FITC / PI double staining method for detecting cell apoptosis: in the early stage of cell apoptosis, phosphatidylserine (phosphalidylserine, PS) located on the inner side of the cell membrane can migrate to the outer side of the cell membrane, and AnnexinV, as a phospholipid-binding protein, can inhibit PS It has a high binding force, so the fluorescence intensity of AnnexinV-labeled fluorescein (FITC) can reflect early apoptotic cells; at the same time, because the PS of necrotic cells is also exposed outside the cell membrane, and is highly stained to PI, the combined use of PI to reject Apoptotic (early apoptosis) and necrotic (late apoptosis) cells can be distinguished by staining. On the scatter diagram of flow cytometry analysis, AnnexinV and PI double nega...

Embodiment 3C3

[0180] Effect of embodiment 3C3G on the mitochondrial membrane potential of HaCaT cells after UVB irradiation

[0181] 1. Mitochondrial membrane potential (Δψm) detection—JC-1 staining flow cytometry detection

[0182] The principle of JC-1 detecting the change of mitochondrial membrane potential: JC-1 is a cationic lipid fluorescent dye, which has two states of monomer and polymer. exist in multimeric form. In normal cells, due to the polarity of the mitochondrial membrane potential (Δψm), JC-1 is rapidly absorbed into the mitochondria depending on the polarity of Δψm, and forms multimers in the mitochondria due to its increased concentration, and the multimers emit The light is red fluorescence, which can be detected by the red (FL-2) channel of the flow cytometer; when cells undergo apoptosis, the mitochondrial membrane potential is depolarized, resulting in the release of JC-1 from the mitochondria, reducing its concentration , so it exists in the form of a monomer and...

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Abstract

The invention discloses a protection effect of cyanidin-3-glucoside (C3G) for UVB (ultraviolet B)-induced cell injury. The protection effect particularly includes that generation of ROS (reactive oxygen species) of HaCaT cells due to UVB can be reduced by the C3G, accordingly, antioxidant effects can be realized, DNA (deoxyribonucleic acid) can be protected against injury, the survival rate of the HaCaT cells can be increased after the UVB irradiates on the HaCaT cells, and apoptosis reducing effects and the like can be realized. The protection effect has the advantages that the oxidation injury of the selected representative HaCaT cells which are cultivated in vitro is induced by means of UVB irradiation, light injury models of epidermal cells due to ultraviolet rays are imitated, then the C3G is used as a light injury protector, effects and relevant channels of the C3G for relieving UVB injury of the HaCaT cells are evaluated, the protection effect, action mechanisms and the safety of the C3G for light injury of skins are investigated, and accordingly theoretical foundation is laid for clinically effectively preventing and treating light injury of the skins and studying ultraviolet radiation injury protection action mechanisms of natural plant antioxidants.

Description

technical field [0001] The invention belongs to the technical field of cosmetics. More specifically, it relates to the protective effect of cyanidin-3-glucoside on UVB-induced damage of HaCaT cells. Background technique [0002] With the serious destruction of the ozone layer in the atmosphere, the intensity of ultraviolet (ultraviolet, UV) radiation has increased, and living standards have improved, and opportunities for outdoor sports and sunbathing have increased, resulting in people receiving excessive ultraviolet radiation, resulting in a gradual increase in photodamaged skin diseases . The intensity of ultraviolet rays (ultraviolet, UV) irradiating on the ground increases, and the ultraviolet rays that cause human skin damage are mainly medium-wave ultraviolet (ulrtavioletB, UVB) and long-wave ultraviolet (ulrtavioletA, UVA), and the damage degree of the former to the skin is about 100%. It can cause acute damage such as sunburn and chronic cumulative damage such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61K31/7048A61Q17/04A61P17/16A61Q19/08A61P39/06
Inventor 白卫滨邓列华胡云峰何咏马月瑭吴实孙建霞焦睿
Owner JINAN UNIVERSITY
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