Preparation method for mixed three templates molecularly imprinted solid phase extraction column and application thereof

A technology of solid-phase extraction column and template molecule, which is applied in the preparation of mixed three-template molecularly imprinted solid-phase extraction column and the preparation of molecularly imprinted polymer, which can solve the problems of high selectivity, high adsorption capacity, and inability to display.

Active Publication Date: 2015-11-11
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The preparation of most MIPs uses a single template molecule, so the synthesized polymer cannot have high affinity for multiple analytes at the same time
However, in actual detection, there is often not a single analyte, so the adsorption of MIPs synthesized by a single template to other compounds of the same family cannot show high adsorption capacity and high selectivity.

Method used

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  • Preparation method for mixed three templates molecularly imprinted solid phase extraction column and application thereof
  • Preparation method for mixed three templates molecularly imprinted solid phase extraction column and application thereof
  • Preparation method for mixed three templates molecularly imprinted solid phase extraction column and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 A preparation method of a mixed three-template molecularly imprinted solid-phase extraction column

[0067] In this example, the preparation of the mixed three-template molecularly imprinted solid-phase extraction column was carried out according to the following sequence of steps:

[0068] ⑾ dissolve

[0069] Dissolve 1 mol of bifentriazole, 1 mol of cyhalothrin and 1 mol of atrazine in 550 L of porogen acetonitrile, and then add 18 mol of functional monomer α-methacrylic acid to obtain A1 ;

[0070] ⑿Prepolymerization

[0071] Put A1 at a frequency of 200Hz for 60min and put it in a refrigerator at 4°C for 6h to obtain B1;

[0072] ⒀ Polymerization

[0073] Add 45 mol of cross-linking agent ethylene glycol dimethacrylate and 2 mol of initiator azobisisobutyronitrile to B1, after ultrasonication at a frequency of 200 Hz for 30 min, inject high-purity nitrogen gas for 10 min, and seal the container to obtain C1;

[0074] Place C1 at 60°C and react in a ...

Embodiment 2-6

[0082] Example 2-6 Preparation method of mixed three-template molecularly imprinted solid-phase extraction column

[0083] Examples 2-6 are respectively a preparation method of a mixed three-template molecularly imprinted solid-phase extraction column. The preparation process is similar to that of Example 1, the only difference is that the corresponding technical parameters in the preparation process are different, and the specific results See Table 1 .

[0084] Table 1 Technical parameters during the preparation of mixed triple-template molecularly imprinted solid-phase extraction columns

[0085]

[0086] Examples 2-6 have simple preparation methods, easy control of the process, and low cost. The imprinted polymer can be directly loaded into the column without grinding. The column has good stability, high strength, acid and alkali resistance, and the imprinted polymer in the column has specific spatial positions. , can achieve specific adsorption.

Embodiment 7

[0087] Example 7 Selection of Functional Monomer and Screening Test of Template Molecule and Functional Monomer Ratio

[0088] In this example, the selection of functional monomers and the ratio of template substances to functional monomers were explored during the polymerization process. In this embodiment, ultraviolet spectroscopy is used to scan the ultraviolet spectrum of the mixed three-template substance-MAA system and the mixed three-template substance-AM system.

[0089] Prepare a certain concentration of MAA-acetonitrile solution (MAA is α-methacrylic acid) and AM-acetonitrile solution (AM is acrylamide) and mixed three-template substance-acetonitrile solution, and prepare a mixed acetonitrile solution of functional monomer and template substance , so that the molar concentration ratio is 1:0, 1:2, 1:4, 1:6, 1:8, 1:10 respectively, and the ultraviolet spectrum scanning is carried out between the wavelength 190-300nm.

[0090] (1) If the template substance does not ...

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Abstract

The invention discloses a preparation method for a mixed three templates molecularly imprinted solid phase extraction column and application thereof, and the preparation method comprises the steps of performing dissolving, prepolymerization, and polymerization in sequence for the adopted three template substances, and eluting template substances, drying and filling a column to obtain the mixed three templates molecularly imprinted solid phase extraction column. The preparation process is simple, easy to control; a prepared mixed three templates molecularly imprinted polymer has good stability, high strength, good uniformity, acid and alkali resistance, high specific adsorption efficiency for substances such as triazole, pyrethroid and triazine, no need to grind, and can be directly filled to form the column. The invention also provides the application of the extraction column absorbing the substances such as the triazole, the pyrethroid and the triazine. The method provided by the invention is suitable for preparing the mixed three templates molecularly imprinted solid phase extraction column; a prepared product can be further used for purification and enrichment of samples when pesticide multi-residues of the triazole, the pyrethroid and the triazine in food, feed and other samples are being analyzed.

Description

technical field [0001] The invention belongs to the fields of chemical synthesis, analytical chemistry, supramolecular chemistry and food safety, and relates to the preparation and application of a molecularly imprinted polymer, in particular to a preparation method of a three-template molecularly imprinted solid-phase extraction column and its application. Background technique [0002] Tebuconazole, bifentriazole and diniconazole are all triazole fungicides, which are broad-spectrum systemic fungicides, mainly used for the prevention and treatment of plant diseases, and have the effect of killing or inhibiting the growth of pathogenic microorganisms. Its high efficiency, broad-spectrum, low toxicity and long-term effect are widely used in the control of crops and other pests. At the same time, the residues of such pesticides in crops, soil and water also have negative effects on biological safety and human health. [0003] Tetramethrin, cyhalothrin and perfluthrin all belo...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30B01J20/281B01D15/08C08F220/06C08F222/14C08J9/28G01N1/34G01N1/40G01N30/08
Inventor 高文惠赵春娟高林何丛薇
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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