Mucus treating fluid and application thereof

A technology for treating liquid and mucus, which is applied in the field of cell biology, can solve the problems of variability, instability, and easy failure, and achieve the effects of not being easy to deteriorate, responding quickly, and cracking quickly

Inactive Publication Date: 2015-11-25
XIAMEN BIOTIME BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is more thorough than the filtration method, and can remove mucus and impurities very effectively, but the disadvantage is the same as the first method, the cells wrapped in mucus cannot be separated, and it is not suitable for sputum liquid base; 3. Using disulfide For threitol lysis, add dithiothreitol directly to the preservation solution or add dithiothreitol before cell sheeting to achieve the effect of lysing mucus
The disadvantage of this method is that dithiothreitol is unstable, easily decomposed, needs to be protected from li

Method used

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  • Mucus treating fluid and application thereof
  • Mucus treating fluid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Embodiment 1: Preparation of mucus treatment liquid

[0027] raw material:

[0028] Sodium chloride 9.0g;

[0029] Tris buffer 0.1M;

[0030] Disodium edetate 3.0g;

[0031] Triton-100 3.0g;

[0032] Methylcysteine ​​5.0g;

[0033] Make up to 1000ml with double distilled water.

[0034] Preparation:

[0035] After weighing and measuring according to the above ingredients, stir to dissolve.

[0036] Using the mucus treatment solution prepared in this example to treat the pleural and ascites cell specimens with mucus, it was observed that there was very little mucus after the disc, basically not covering the cells and affecting the interpretation, and all the cells could be clearly displayed under the microscope.

Example Embodiment

[0037] Embodiment 2:: preparation of mucus treatment liquid

[0038] raw material:

[0039] Sodium chloride 5.0g;

[0040] Tris buffer 0.01M;

[0041] Disodium edetate 1.0g;

[0042] Triton-1001.0g;

[0043] Methylcysteine ​​1.0g;

[0044] Make up to 1000ml with double distilled water.

[0045] Preparation method: with embodiment 1.

[0046] Using the mucus treatment solution prepared in this example to treat the pleural and ascites cell specimens with mucus, it was observed that there was very little mucus after the disc, basically not covering the cells and affecting the interpretation, and all the cells could be clearly displayed under the microscope.

Example Embodiment

[0047] Embodiment 3: Preparation of mucus treatment liquid

[0048] raw material:

[0049] Sodium chloride 7.0g;

[0050] Tris buffer 0.05M;

[0051] Disodium edetate 2.0g;

[0052] Triton-1000.5g;

[0053] Methylcysteine ​​4.0g;

[0054] Make up to 1000ml with double distilled water.

[0055] Preparation method: with embodiment 1.

[0056] The cervical cell specimens with mucus were treated with the mucus treatment solution prepared in this example. It was observed that there was very little mucus after the disc, which basically did not cover the cells and affect the interpretation, and all the cells could be clearly displayed under the microscope.

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PUM

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Abstract

The invention discloses mucus treating fluid and the application thereof. The mucus treating fluid is composed of 5-9 g/L sodium chloride, 0.01-0.1 M Tris buffer solution, 0.1-3 g/L dispersing agent, surface active agent with a volume fraction of 0.1-0.3%, 1-5 g/L mucus decomposing agent and double distilled water. The mucus treating fluid can be stored at the normal temperature without going bad easily. The mucus treating fluid is compatible with acid or alkaline solutions, free of toxicity, specific and efficient. By the adoption of the mucus treating fluid, mucus splitting decomposition is quick, the fluid is mild to cells, and cell damage during mucus degradation is avoided.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a mucus treatment liquid and its application. Background technique [0002] When taking cervical cell samples or sputum cells clinically, the samples often carry a large amount of mucus, which will wrap the cells and affect the tiling effect of the cells on the glass slide. The mucus adheres to the slide, resulting in complex background and readable cells. limited, affecting the accuracy of the results. In view of this problem, there are four kinds of clinically adopted solutions at present: 1. Use a filter screen to filter, block the large pieces of mucus on the filter screen, and reduce the area where the mucus adheres to the glass slide. The main disadvantage of this method is that the large mucus is removed, and small The mucus still affects the film production, and the mucus block will be filtered out directly, and a large number of cells wrapped will also be filtered ...

Claims

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Application Information

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IPC IPC(8): A01N1/00G01N1/30
Inventor 张国锋颜珊张恩思
Owner XIAMEN BIOTIME BIOTECHNOLOGY CO LTD
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