Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ST cell culture medium

A culture medium and cell technology, applied in the field of biomedicine, can solve the problems of inconsistent ingredients, great differences in quality, and different batch numbers.

Inactive Publication Date: 2015-11-25
上海源培生物科技股份有限公司
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ST cells are cultured by animal serum. Individual animals are different, and the origin and batch number of serum are different. The quality of each batch varies greatly, and its components cannot be kept consistent. Bacteria and viruses are prone to be produced, which is not conducive to cell culture. Although the cost of using animal serum is low, It is not easy to cultivate pure cells, which is not conducive to long-term development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ST cell culture medium
  • ST cell culture medium
  • ST cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention will be further elaborated below.

[0028] A kind of ST cell culture medium, in terms of final concentration, the formula is as follows: L-arginine hydrochloride 1mg / L-1000mg / L, 2-mercaptoethanol 0.1mg / L-200mg / L, meso-inositol 0.01 mg / L-10mg / L, monoethanolamine hydrochloride 1mg / L-1000mg / L, plant hydrolyzate 50mg / L-5000mg / L, 4-hydroxyethylpiperazineethanesulfonic acid 1000mg / L-4000mg / L, Sodium chloride 1000mg / L-7000mg / L and glycine 1mg / L-100mg / L. The present invention adopts the plant hydrolyzate to cultivate ST cells, because the components in the plant hydrolyzate are consistent and can reduce the virus produced in the ST cell culture process, increase the survival rate and accelerate the proliferation speed. 2-mercaptoethanol acts as an antioxidant in the culture medium, 4-hydroxyethylpiperazineethanesulfonic aci...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biological medicines, and in particular relates to an ST cell culture medium. Based on the final concentration, the ST cell culture medium is prepared from the following components: 1mg / L-1000mg / L L-arginine monohydrochloride, 0.1mg / L-200mg / L 2-mercaptoethanol, 0.01mg / L-10mg / L meso-inositol, 1mg / L-1000mg / L aminoethanol hydrochloride, 50mg / L-5000mg / L plant hydrolysate, 1000mg / L-4000mg / L 4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid, 1000mg / L-7000mg / L sodium chloride, and 1mg / L-100mg / L glycine. With the ST cell culture medium, viruses generated during the culture process of ST cells are reduced, the survival rate is increased, and the cell proliferation speed is accelerated.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a culture medium. Background technique [0002] ST cells are pig testicular (swinetestis) cells that grow adherently in vitro, proliferate slowly during culture, and tend to form clumps during digestion and passage. ST cells are sensitive to various viruses, such as classical swine fever virus (CSFV), porcine parvovirus (PPV), pseudorabies virus, etc. At present, ST cells are cultured by animal serum. Individual animals are different, and the origin and batch number of serum are different. The quality of each batch varies greatly, and its components cannot be kept consistent. Bacteria and viruses are prone to be produced, which is not conducive to cell culture. Although the cost of using animal serum is low, It is not easy to cultivate relatively pure cells, which is not conducive to long-term development. Contents of the invention [0003] The object of the present invention is to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 温韬陈杰贾人初
Owner 上海源培生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com