Unlock instant, AI-driven research and patent intelligence for your innovation.

Ovine chlamydia LAMP detection kit

A detection kit, technology for sheep chlamydia, applied in the field of kits

Inactive Publication Date: 2015-11-25
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now the market lacks a simple, fast, sensitive and easy-to-promote LAMP detection kit for Chlamydia ovis in clinical samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ovine chlamydia LAMP detection kit
  • Ovine chlamydia LAMP detection kit
  • Ovine chlamydia LAMP detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0052] Embodiment of the present invention: Chlamydia goat LAMP detection kit, which comprises 1 μL of primer F31 μL with a concentration of 0.5 μmol / L, 1 μL of primer B3 with a concentration of 0.5 μmol / L, 2 μL of primer FIP with a concentration of 1 μmol / L, and 2 μL of primer FIP with a concentration of 1 μmol / L 2 μL of primer BIP, 1 μL of BstDNA polymerase, 13 μL of LAMP reaction buffer, 1.5 μL of template, 1 μL of fluorescent chromogenic solution, and ddH 2 O supplemented to 2.5 μL, paraffin oil 1.5-2 μL, 2 μL negative control and 2 μL positive control; the sequence of the primer F3 is: 5'-acaagcctagaggatacaag-3; the sequence of the primer B3 is: 5'-gttgagcgatgcggatagtg- 3; the sequence of the primer FIP is: 5'-agtatccgtagcagcctctgtgaacgtccgccaactttcc-3; the sequence of the primer BIP is 5'-gtctagcactctcttacagatgtgaccagtttacgccaatg-3'; the negative control is Chlamydia non-specific DNA fragment; the positive control is the standard Chlamydia MOMP gene LAMP product; in the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an ovine chlamydia LAMP detection kit comprising 1 mu L of a primer F3 with the concentration of 0.5 mu mol / L, 1 mu L of a primer B3 with the concentration of 0.5 mu mol / L, 2 mu L of a primer FIP with the concentration of 1 mu mol / L, 2 mu L of a primer BIP with the concentration of 1 mu mol / L, 1 mu L of Bst DNA polymerase, 13 mu L of an LAMP reaction buffer, 1.5 mu L of a template, 1 mu L of a fluorescent developing solution, the balance of ddH2O to be 2.5 mu L, 10-15 mu L of paraffin oil, 2 mu L of a negative control and 2 mu L of a positive control. Four specific primers are designed in conserved domains of chlamydia MOMP gene sequences according to the principle of LAMP primer design, the kit is established to quickly detect ovine chlamydia MOMP genes, and the kit has the characteristics of quickness, high sensitivity, high specificity, easiness in popularization and the like.

Description

technical field [0001] The invention relates to a kit, in particular to a detection kit for Chlamydia goat LAMP. Background technique [0002] Chlamydiosis in sheep is caused by Chlamydia psittaci ( C . psittaci ) and ruminant Chlamydia ( C . pecorum ) caused by an infectious disease. Clinically, Chlamydia psittaci is characterized by fever, miscarriage and weak lambs. Ruminant Chlamydia causes polyarthritis, conjunctivitis and enteritis in sheep, and is one of the main diseases that endanger sheep. The disease can also cause significant economic losses to the sheep industry. Sheep chlamydiosis exists in some sheep breeding bases, professional sheep raising households and farmers' flocks in my country, which seriously endangers the health of sheep. The market lacks a kind of LAMP detection kit that can carry out simple, fast, sensitive, easy promotion to the Chlamydia ovis of clinical sample at present. Contents of the invention [0003] The purpose of the present ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/689
Inventor 杨茂生杨莉史开志吴玙彤杨丽娟谢丽丽吴位珩龙明珠张宁新
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY