Method for utilizing Raman spectrum to rapidly detect free amino acid content of yellow rice wine
A free amino acid and Raman spectroscopy technique is applied in the field of rapid detection of free amino acid content in rice wine by using Raman spectroscopy, which can solve the problems of complex operation, high detection cost and low accuracy, and achieves simple operation, low detection cost and high detection rate. short time effect
Inactive Publication Date: 2015-11-25
SHANGHAI INST OF TECH
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[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a method for rapidly detecting the content of free amino acids in yellow rice wine by using Raman spectroscopy. Technical problems of high cost, complicated operation, and low accuracy
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Embodiment 1
[0049] A method for rapidly detecting free amino acids in yellow rice wine by using Raman spectroscopy, comprising the steps of:
[0050] (1) Model establishment of free amino acids in rice wine
[0051] ①. The Raman spectrum of the rice wine sample in the modeling set
[0052] The Raman spectrum of the rice wine sample in the modeling set was collected by a Raman spectrometer, and a clear Raman spectrum was obtained;
[0053] ②. Amino acid content of rice wine samples in modeling set
[0054] The content of free amino acids in the rice wine samples of Modeling Ji was determined by high performance liquid chromatography. Take 1 mL of rice wine into a 10 mL centrifuge tube, add 4 mL of trichloroacetic acid, shake well, and sonicate twice for 15 minutes each time (with a few minutes in between). Leave it for 1h after ultrasonication, filter with two layers of filter paper, take 1mL of the filtrate in a 1.5mL centrifuge tube, centrifuge at 10000r / min for 10min, take 0.5mL of th...
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Provided is a method for utilizing a Raman spectrum to rapidly detect the free amino acid content of yellow rice wine. The method comprises the steps that the yellow rice wine is collected to serve as a modeling set sample for modeling; the Raman spectrum information of the modeling set yellow rice wine sample is acquired by using a Raman spectrometer to obtain a Raman spectrogram of the modeling set yellow rice wine sample; a chemical value Yci of the free amino acid content in the modeling set yellow rice wine sample is detected by adopting a high performance liquid chromatograph; a model is established according to the Raman spectrogram, obtained by the Raman spectrometer, of the modeling set yellow rice wine sample and the free amino acid content detected by the high performance liquid chromatograph; the performance of the model is evaluated by comparing the correlation coefficient r and the modeling standard deviation RMSEC which are obtained through calculation of the chemical value Yci and a predicted value (shown in the description) of the modeling set sample, the Raman spectrogram of a yellow rice wine sample to be detected is obtained according to the Raman spectrum information, acquired by the Raman spectrometer, of the yellow rice wine sample to be detected; the Raman spectrogram is introduced into the established free amino acid model to obtain the free amino acid content of the yellow rice wine sample to be detected. The method is simple in operation and low in detection cost.
Description
technical field [0001] The invention belongs to the field of food analysis, and in particular relates to a detection method, in particular to a method for rapidly detecting the content of free amino acids in rice wine by using Raman spectroscopy. Background technique [0002] Rice wine is a kind of low-alcohol brewed wine that ferments rice and millet, and controls the alcohol content within 14%-20%. Yellow rice wine has high nutritional content and is rich in organic acids, polypeptides and amino acids, among which there are 21 kinds of amino acids, including all 8 kinds of essential amino acids for human body. The importance of free amino acids is not only reflected in its great nutrition, but also in its influence on the taste, quality and flavor of yellow rice wine. Therefore, in order to realize the monitoring of rice wine quality, the content of free amino acids in rice wine should be determined quickly and accurately. [0003] Commonly used amino acid analysis metho...
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Inventor 于海燕张燕谢丽娟刘湘江
Owner SHANGHAI INST OF TECH