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Preparation method of nano-particle material

A technology of nanoparticle and preparation method, which is applied in the field of preparation of nanoparticle materials and can solve problems such as environmental pollution

Inactive Publication Date: 2015-12-02
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The chemical synthesis methods are flexible and diverse, and can be used to prepare nanoparticles of various shapes, but most chemical methods require the introduction of more chemical reagents, which may cause certain environmental pollution problems

Method used

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  • Preparation method of nano-particle material
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  • Preparation method of nano-particle material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 silver nanoparticles

[0026] Weigh 10g of peptone, 5g of yeast and 10g of sodium chloride and dissolve in 1L of deionized water to adjust the pH to about 7. Stored after high temperature and high pressure sterilization. Inoculate the strain Pantoea agglomerans IMH into the obtained 100 mL medium solution with an inoculation loop. Afterwards, the solution was placed in a shaker and cultured with shaking at 30° C. and 150 rpm for 12 hours. Subsequently, 1 mL of the activated bacterial solution was drawn and transferred to 100 mL of sterilized medium, and the solution was placed in a shaker again, and cultured with shaking at 30° C. and 150 rpm for 12 h. Take 50mL logarithmic phase bacteria solution in a centrifuge tube and centrifuge at 8000rpm for 5 minutes. When finished, remove the supernatant and collect the cells at the bottom of the tube. The collected cells were dispersed into 50 mL of sterilized LB medium without NaCl. Add a certain amount of si...

Embodiment 2

[0027] Embodiment 2 gold nanoparticles

[0028] Weigh 10g of peptone, 5g of yeast and 10g of sodium chloride and dissolve in 1L of deionized water to adjust the pH to about 7. Stored after high temperature and high pressure sterilization. Inoculate the strain Pantoea agglomerans IMH into the obtained 100 mL medium solution with an inoculation loop. Afterwards, the solution was placed in a shaker and cultured with shaking at 30° C. and 150 rpm for 12 hours. Subsequently, 1 mL of the activated bacterial solution was drawn and transferred to 100 mL of sterilized medium, and the solution was placed in a shaker again, and cultured with shaking at 30° C. and 150 rpm for 12 h. Take 50mL logarithmic phase bacteria solution in a centrifuge tube and centrifuge at 8000rpm for 5 minutes. When finished, remove the supernatant and collect the cells at the bottom of the tube. The collected cells were dispersed into 50 mL of sterilized PBS buffer. Add a certain amount of chloroauric acid...

Embodiment 3

[0029] Embodiment 3 palladium nanoparticles

[0030] Weigh 10g of peptone, 5g of yeast and 10g of sodium chloride and dissolve in 1L of deionized water to adjust the pH to about 7. Stored after high temperature and high pressure sterilization. Inoculate the strain Pantoea agglomerans IMH into the obtained 100 mL medium solution with an inoculation loop. Afterwards, the solution was placed in a shaker and cultured with shaking at 30° C. and 150 rpm for 12 hours. Subsequently, 1 mL of the activated bacterial solution was drawn and transferred to 100 mL of sterilized medium, and the solution was placed in a shaker again, and cultured with shaking at 30° C. and 150 rpm for 12 h. Take 50mL logarithmic phase bacteria solution in a centrifuge tube and centrifuge at 8000rpm for 5 minutes. When finished, remove the supernatant and collect the cells at the bottom of the tube. The collected cells were dispersed into 50 mL of sterilized PBS buffer. A certain amount of palladium nitra...

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Abstract

The invention provides a culture medium prepared from peptone, yeast and NaCl, wherein strains cultured in the culture medium are pantoea agglomerans belonging to IMH cells. After being collected, thallus cells are dispersed to different cultivation systems according to the difference among nano-particles required to be synthesized, metal ions are added to meet the requirement of a proper concentration, reactions are carried out for several hours, and separation and collection are carried out according to different reaction times, so as to obtain metal nano-particle materials of different particle diameters. The whole process is environmentally friendly, simple and convenient to operate, and low in cost; the obtained nano-particle materials have wide application prospects in the aspects of catalysis, adsorption, detection and the like.

Description

technical field [0001] The invention provides a method for preparing nanoparticle materials, which belongs to the technical field of nanomaterial synthesis. technical background [0002] Nanomaterials refer to materials with special properties that have at least one dimension in the three-dimensional space in the range of nanoscale or constituted by them as basic units. When the particle size of the material enters the nanometer level, it has quantum size effect, small size effect, surface effect, and quantum tunnel effect, so it shows many unique physical and chemical properties. It is widely used in catalysis, environmental protection, energy, medicine, etc. The field has broad application prospects. [0003] At present, the synthesis methods of nanoparticles can be generally divided into two ways: "top-down" and "bottom-up". The "top-down" method is a process of changing from large to small. Large objects are transformed into nano-sized particles through physical method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 景传勇刘文婧杜晶晶
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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