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Microorganism aerobiotically reducing arsenic high-effectively

A high-efficiency, pentavalent arsenic technology, applied in the field of microorganisms, to achieve the effect of deepening and broadening understanding

Inactive Publication Date: 2015-10-14
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on microorganisms that can reduce arsenic under aerobic conditions.

Method used

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  • Microorganism aerobiotically reducing arsenic high-effectively
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  • Microorganism aerobiotically reducing arsenic high-effectively

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1 Isolation of high-efficiency aerobic arsenic-reducing bacteria: the present invention uses trypsin, yeast, glucose compound medium (TYEG) to isolate aerobic arsenic-reducing bacterial strains. Take 1g and 20mL of soil samples from areas with high arsenic pollution containing 5mmol L-1 or 10mmol L -1 Add TYEG medium (pH 7.0) of trisodium arsenate into a 50mL Erlenmeyer flask, place the Erlenmeyer flask in a constant temperature shaking incubator, and incubate at 28°C for 48 hours with shaking (150rpm).

[0022] Isolation and purification of the aerobic arsenic-reducing strains were performed using conventional microbial methods. Every 24 hours, take 1 mL of the aforementioned culture solution and transfer it into the sterilized liquid medium of the same concentration. Repeat this operation twice, take out 1mL of the culture solution, and use fresh TYEG medium to make a concentration gradient dilution to 10-6, and use the method of flat plate coating to evenly ...

Embodiment 2

[0026] Example 2 Study on the arsenic reduction characteristics of highly efficient aerobic arsenic reducing bacteria: the minimum growth inhibitory concentration (MIC) is used to characterize the arsenic resistance of the bacteria. The minimum growth inhibitory concentration (MIC) is defined as the minimum concentration of trivalent arsenic or pentavalent arsenic that inhibits the growth of bacteria after 48 hours of culture. Prepare 0-80mmol L -1 As(III) (NaAsO2) and 0-300mmol L -1 As(V) (NaAsO 4 ) TYEG liquid medium, inoculated with the same concentration of bacterial liquid, cultured at 30°C with constant temperature shaking (150rpm) for 48h, and measured OD 600 value.

[0027] Research on the reducing characteristics of bacteria: under aerobic conditions, use 1mmol L -1 As(V) TYEG liquid medium, inoculated with the target strain, cultured at 30°C with constant temperature shaking (150rpm). Every 2-4 hours, take a sample of the suspension and pass it through a 0.22 μm...

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Abstract

The invention relates to a microorganism aerobiotically reducing arsenic high-effectively, which is separated from a high-arsenic pollution region in Inner Mongolia, China. 16S rDNA identification proves that the microorganism belongs to pantoea agglomerans and is named Pantoea sp.IMH. A bacterial strain of the microorganism is assigned the accession number CGMCC No.8825. The microorganism is wide in pH and temperature ranges of growth, is very high in tolerance on trivalent arsenic and pentavalent arsenic, and can reduce more than 90% of the pentavalent arsenic in 36 h under an aerobiotical culture condition.

Description

technical field [0001] The invention relates to a microorganism, in particular to a microorganism capable of efficiently reducing arsenic under aerobic conditions. technical background [0002] Arsenic is a teratogenic and carcinogenic substance that widely exists in nature. Arsenic in natural water bodies is mostly inorganic arsenic, mainly in trivalent and pentavalent forms. Among them, trivalent arsenic has strong mobility, and its toxicity is 25-60 times that of pentavalent arsenic. Although arsenic is toxic, some microorganisms in the natural environment can survive in a high-arsenic environment and derive a variety of arsenic metabolism mechanisms, including arsenic oxidation, reduction, and methylation. According to the different mechanisms of arsenic metabolism, these microorganisms can be divided into chemoautotrophic arsenic oxidizing microorganisms, heterotrophic arsenic oxidizing microorganisms, dissimilatory arsenic reducing microorganisms, and arsenic detoxif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/40
CPCA62D3/02A62D2101/43C12N1/205C12R2001/01
Inventor 景传勇田海霞
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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