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A stain of Pantoea agglomerans and use thereof

A Pantoea agglomerans, applied technology, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the undiscovered Pantoea agglomerans and other problems, achieve strong Fe(III) reduction and humic acid reduction capabilities, The screening method is simple and the electron utilization spectrum is wide

Active Publication Date: 2009-01-28
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no report has been found on Pantoea agglomerans with Fe(III) respiration and humic acid respiration activities

Method used

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  • A stain of Pantoea agglomerans and use thereof
  • A stain of Pantoea agglomerans and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 Enrichment, separation and purification of Pantoea agglomerans MFC3 of the present invention

[0019] Take 5g ancient forest soil sample in 100mL liquid separation medium. Each liter of liquid separation medium contains 1.0g glucose (as an electron donor), 0.412g AQDS (2,6-anthraquinone disulfonate, as an electron acceptor), 2.5g NaHCO 3 , 0.25g NH 4 Cl, 0.678g NaH 2 PO 4 2H 2 O, 0.1g KCl, 10.0mL vitamin solution, 10.0mL trace element solution. Among them, the vitamin solution contains 2.0mg biotin, 2.0mg folic acid, B610.0mg vitamin, 5.0mg thiamine, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium pantothenate and 0.1mg vitamin per liter of deionized water. B12, 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid; the trace element solution contains 1.5g nitrilotriacetic acid, 3.0g MgSO per liter of deionized water 4 ·7H 2 O, 0.5g MnSO 4 ·H 2 O, 1.0g NaCl, 0.1g FeSO 4 ·7H 2 O, 0.1gCoCl 2 ·6H 2 O, 0.1g CaCl 2 , 0.1g ZnSO 4 ·7H 2 O, 0.01g CuSO 4 ·5H ...

Embodiment 2

[0034] Example 2 Reduction activity of Pantoea agglomerans MFC3 on goethite with glucose as electron donor

[0035] Medium A formula: 2.25g of goethite per liter of deionized water, NaHCO 3 2.5g, NH 4 Cl0.25g, NaH 2 PO 4 2H 2 O 0.678g, KCl 0.1g, glucose 1.0g, vitamin solution and trace element solution 10.0mL each, sterilized at 121°C for 20 minutes, glucose was sterilized alone, and mixed with other ingredients after sterilization. Wherein the composition of vitamin solution and trace element solution is the same as that of the separation medium described in Example 1.

[0036] Preparation of bacterial suspension: use an inoculation loop to pick a circle of activated bacterial strains in 200mL beef extract peptone liquid medium, and cultivate it in a shaker (30°C, 180 rpm) for 16 hours, so that the number of bacteria reaches the exponential growth phase, Collect the bacterial cells by centrifugation at 8000 rpm, pour off the supernatant, and suspend the precipitated bact...

Embodiment 3

[0041] Example 3 Reduction activity of Pantoea agglomerans MFC3 on lepidocite with glucose as electron donor

[0042] Medium B formula: per liter of deionized water containing 2.25g lepidocerite, NaHCO 3 2.5g, NH 4 Cl0.25g, NaH 2 PO 4 2H 2 O 0.678g, KCl 0.1g, glucose 1.0g, each 10.0mL of vitamin solution and trace element solution (the components of vitamin solution and trace element solution are the same as the separation medium described in Example 1), sterilized at 121°C for 20 minutes, glucose alone Sterilized, mixed with other ingredients after sterilization.

[0043] Preparation of bacterial suspension: use an inoculation loop to pick a circle of activated bacterial strains in 200mL beef extract peptone liquid medium, and cultivate it in a shaker (30°C, 180 rpm) for 16 hours, so that the number of bacteria reaches the exponential growth phase, Collect the bacterial cells by centrifugation at 8000 rpm, pour off the supernatant, and suspend the precipitated bacterial ...

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Abstract

The invention discloses a pantoea agglomerans and application thereof, and also discloses a screening method of the pantoea agglomerans; the invention obtains the pantoea agglomerans (MFC3) by separation and purification from the soil sample of an ancient forest of Zhaoqing in Guangdong, which is preserved in China culture collection and management committee general microorganisms center, with the preservation number of CGMCC2453; the invention has stronger reducing capacity of Fe(III) or humic acid and has the advantages that the electronic utilization spectrum is wide, the iron oxides with high crystallinity can be reduced, the screening method is simple, and has the possibility of industrial production.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and environmental biotechnology, and specifically relates to a strain of Pantoea agglomerans (Pantoea agglomerans MFC3), its screening method and its application in Fe(III) reduction and humic acid reduction. technical background [0002] Fe(III) respiration and humic acid respiration are two forms of microbial respiration metabolism that are ubiquitous in anaerobic environments. Fe(III) respiration refers to the metabolic process in which microorganisms use Fe(III) as the electron acceptor at the end of the respiratory chain to transfer electrons on the respiratory chain and obtain energy from the electron transfer process for growth. Humic acid respiration refers to the process in which microorganisms use humic acid as the only terminal electron acceptor to accept electrons provided by organic acids and other substances, couple energy generation, and support the growth of bacteria....

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P9/00C12P13/00C12R1/01
Inventor 周顺桂武春媛王跃强庄莉李芳柏
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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