Entomopathogenic nematode HbSD and insecticide thereof, preparation method and application

A technology for entomopathogenic nematodes and insects, applied in the direction of insecticides, botanical equipment and methods, applications, etc., can solve the problem of high cost, achieve the effects of low cost, simplified preparation and use methods, and improved insecticidal efficiency

Inactive Publication Date: 2015-12-09
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the other hand, the suspension formulation provided by the present invention solves the problems of high cost caused by adding multiple auxiliary agents and protective agents in the production process of biopesticides

Method used

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  • Entomopathogenic nematode HbSD and insecticide thereof, preparation method and application
  • Entomopathogenic nematode HbSD and insecticide thereof, preparation method and application
  • Entomopathogenic nematode HbSD and insecticide thereof, preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: the preparation method of entomopathogenic nematode HbSD suspension preparation

[0024] The first entomopathogenic nematode HbSD insecticide is a suspension preparation, and its preparation method comprises:

[0025] The preparation method of the entomopathogenic nematode HbSD suspension preparation: put a layer of filter paper at the bottom of the plastic box, select 100 of the last instar Tenebrio molitor larvae that grow healthy and place them on the filter paper, and use a pipette gun to absorb HbSD nematodes with a concentration of 1000 heads / ml Drop the suspension on the last instar Tenebrio molitor, cover it, and check the color change of the insect body after 4-5 days at room temperature: on the sixth day, the color of Tenebrio molitor normally infected with entomopathogenic nematodes turns reddish-brown, and discard the color. Normal Tenebrio molitors were continued to be cultured, and Tenebrio molitors infected with the entomopathogenic nematode...

Embodiment 2

[0029] Example 2: Preparation of Entomopathogenic Nematode HbSD Entomicide

[0030] The second entomopathogenic nematode HbSD insecticide is an insecticide, and its preparation method comprises:

[0031] The preparation method of entomopathogenic nematode HbSD carcasses: on the first day, the harvested larvae of entomopathogenic nematode HbSD infection stage are used to infect the last instar Tenebrio molitor larvae. On the 6th day, select the Tenebrio molitor whose color has changed to reddish brown, and continue to cultivate. On the 9th day, the dead insects are harvested, placed in a 50-100mL centrifuge tube, and stored at 10-14°C for later use.

[0032] The survival rate of the entomopathogenic nematode HbSD is greater than 95%. The carcasses are the last instar Tenebrio molitor larvae infected by HbSD, and each carcass contains at least 5000 entomopathogenic nematode HbSD.

[0033] Two kinds of HbSD nematode insecticides of the present invention: nematode suspension prep...

Embodiment 3

[0034] Embodiment 3: The insecticidal property experiment of entomopathogenic nematode HbSD

[0035] The tested nematode strains: 4 species of bacteriophage Heterobacterium nematodes HbSD, HbI, HbII and HbIII.

[0036] Insects tested: Larvae of the last instar wax moth

[0037] Test method: Indoor bioassay method, that is, to test the toxicity of the suspension of 4 kinds of Heterobacteria genus nematodes to the last instar wax moth larvae, and count the mortality after 6 days. The control group received no nematodes.

[0038] Result and analysis: the insecticidal activity of four species of Heterobacteria nematodes against last-instar wax moth larvae is as follows: figure 1 As shown, among the four tested nematode strains (HbSD, HbI, HbII, HbIII), the entomopathogenic nematode HbSD of the present invention has the highest insecticidal efficacy and strong reproductive ability. HbSD was chosen for further virulence assays.

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Abstract

The invention discloses an entomopathogenic nematode HbSD and insecticide thereof, a preparation method and an application. The preparation method of a suspension liquid preparation comprises the steps that last instar flour weevil larvas are infected with the HbSD, red dead insects are obtained on the sixth day, the HbSD continues to be cultured to the tenth day, the HbSD crawls out of dead insect corpses into water, and suspension liquid is obtained; Triton X-100 is added to the suspension liquid, and the entomopathogenic nematode HbSD suspension liquid preparation is prepared. The preparation method of an insect corpse agent comprises the steps that last instar flour weevil larvas are infected with the HbSD, reddish brown dead insects are obtained on the sixth day, and the HbSD continues to be cultured to the ninth day to obtain the insect corpse agent. The suspension liquid preparation and the insect corpse agent have the advantages of being stable in effect, easy to produce, low in cost, free of pollution, high in injurious insect killing activity and the like.

Description

technical field [0001] The invention relates to biological insecticides, in particular to entomopathogenic nematode HbSD, its insecticides, preparation methods and applications. Background technique [0002] Entomopathogenic nematodes are a type of insect-parasitic nematodes that carry symbiotic bacteria in their bodies. As a new type of biopesticide in the world, this type of nematode has a wide range of hosts; it is safe for vertebrates, plants and other non-target organisms, and is conducive to environmental protection; It is easy to carry out large-scale cultivation in vivo or in vitro, and is convenient to use; it can actively find hosts and quickly kill pests; it can be recycled in the environment and can be mixed with a variety of chemical and biological pesticides. played an important role in concealing pests. [0003] Entomopathogenic nematodes belong to the animal kingdom Nematoda, Secernentea, and Rhabditida. So far, there are more than 40 known nematodes that pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N63/00A01N25/04A01P7/04
Inventor 白成龙海波岳建军孙燕芳刘丽平彭正强
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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