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Free cholesterol assay kit and use method thereof

A cholesterol and kit technology, applied in the field of free cholesterol determination kits, can solve the problems of difficult popularization, low accuracy, complicated operation, etc., and achieve the effects of easy popularization, high accuracy and simple operation.

Inactive Publication Date: 2015-12-09
SHAOXING SHENG KANG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently used free cholesterol assay kits have low accuracy, complicated operation, and are not easy to popularize.

Method used

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  • Free cholesterol assay kit and use method thereof
  • Free cholesterol assay kit and use method thereof
  • Free cholesterol assay kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A free cholesterol assay kit, comprising a first detection solution and a second detection solution, the first detection solution comprises polyethylene glycol, ascorbate oxidase, and peroxidase, and the concentration ratio of raw materials is: polyethylene glycol: ascorbic acid Oxidase: peroxidase=2:5:10; The second detection solution comprises 4-aminoantipyridine, peroxidase, cholesterol oxidase, and the raw material concentration ratio is: 4-aminoantipyridine: Peroxidase:cholesterol oxidase=2:10:5.

[0015] A method for using a free cholesterol assay kit, comprising the steps of: firstly preparing a first reagent tube, a second reagent tube, and a third reagent tube; then adding 240 μL of a first detection solution and 3 μL of distilled water into the first reagent tube, mixing them uniformly and Incubate for 5 minutes. At the same time, add 240 μL of the first detection solution and 3 μL of the standard solution to the second reagent tube, mix well and incubate for ...

Embodiment 2

[0020] A free cholesterol assay kit, comprising a first detection solution and a second detection solution, the first detection solution comprises polyethylene glycol, ascorbate oxidase, and peroxidase, and the concentration ratio of raw materials is: polyethylene glycol: ascorbic acid Oxidase: peroxidase=1:4:9; The second detection liquid comprises 4-aminoantipyridine, peroxidase, cholesterol oxidase, and raw material concentration ratio is: 4-aminoantipyridine: Peroxidase:cholesterol oxidase=1:9:4.

[0021] A method for using a free cholesterol assay kit, comprising the steps of: firstly preparing a first reagent tube, a second reagent tube, and a third reagent tube; then adding 240 μL of a first detection solution and 3 μL of distilled water into the first reagent tube, mixing them uniformly and Incubate for 5 minutes. At the same time, add 240 μL of the first detection solution and 3 μL of the standard solution to the second reagent tube, mix well and incubate for 5 minute...

Embodiment 3

[0027] A free cholesterol assay kit, comprising a first detection solution and a second detection solution, the first detection solution comprises polyethylene glycol, ascorbate oxidase, and peroxidase, and the concentration ratio of raw materials is: polyethylene glycol: ascorbic acid Oxidase: peroxidase=3:6:11; The second detection liquid comprises 4-aminoantipyridine, peroxidase, cholesterol oxidase, and raw material concentration ratio is: 4-aminoantipyridine: Peroxidase:cholesterol oxidase=3:11:6.

[0028] A method for using a free cholesterol assay kit, comprising the steps of: firstly preparing a first reagent tube, a second reagent tube, and a third reagent tube; then adding 240 μL of a first detection solution and 3 μL of distilled water into the first reagent tube, mixing them uniformly and Incubate for 5 minutes. At the same time, add 240 μL of the first detection solution and 3 μL of the standard solution to the second reagent tube, mix well and incubate for 5 minu...

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Abstract

The invention discloses a free cholesterol assay kit and a use method thereof. The free cholesterol assay kit comprises a first detection liquid and a second detection liquid, wherein the first detection liquid comprises polyethylene glycol, ascorbic acid oxidase and peroxidase according to the concentration ratio being (1 to 3):(4 to 6):(9 to 11); the second detection liquid comprises 4- aminoantipyrine, peroxidase and cholesterol oxidase according to the concentration ratio being (1 to 3):(9 to 11):(4 to 6). By adopting an enzymic colorimetric method, the free cholesterol assay kit and the use method thereof disclosed by the invention have the advantages of simplicity in operation, high accuracy and convenience in popularization.

Description

technical field [0001] The invention relates to a kit for measuring free cholesterol and its application method. Background technique [0002] Cholesterol in the blood only exists in a limited and narrow range under normal conditions, and since it will rise with liver parenchymal impairment, cholesterol becomes an important item in liver function tests. However, the currently used kits for free cholesterol determination have low accuracy, complicated operation, and are not easy to popularize. Contents of the invention [0003] The object of the present invention is to provide a free cholesterol assay kit and its use method, which is easy to operate, high in accuracy and easy to popularize by enzymatic colorimetry. [0004] In order to achieve the above object, technical scheme of the present invention is: [0005] A free cholesterol assay kit, comprising a first detection solution and a second detection solution, the first detection solution comprises polyethylene glycol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 黄争上
Owner SHAOXING SHENG KANG BIOLOGICAL TECH
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