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Method for significantly improving fir tissue culture production emergence rate

A technology of seedling emergence rate and Chinese fir, which is applied in the field of plant clone tissue culture, can solve the problems of low transplanting survival rate, low multiplication multiple, and uneven differentiation, so as to increase the multiplication multiple, overcome uneven growth, and promote transplanting of seedlings The effect of branches

Active Publication Date: 2015-12-16
广西壮族自治区国有黄冕林场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems of low seedling emergence rate, poor rooting rate, and low transplanting survival rate caused by low multiplication factor, weak seedling stock and irregular differentiation in the prior art, and provides a method for significantly improving the emergence of Chinese fir tissue culture production. rate method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Bud proliferation induction culture

[0026] Inoculate aseptic single buds of Chinese fir about 1.5-2.5cm long on the proliferation induction medium for proliferation induction, and transfer to fresh proliferation induction medium when 2-5 buds germinate at the base, and remove the original buds when transferring Bud tops to promote the growth of new shoots, thus forming clusters of secondary seedlings.

[0027] The formulation of the proliferation-inducing medium is as follows, each liter contains: 1.0 mg of 6BA, 0.1 mg of NAA, 30 g of white granulated sugar, 3.2 g of agar powder, and the balance is MS medium, adjusting the pH value before sterilization 5.85 (the preparation method is to mix the above ingredients evenly, adjust the pH value, and then sterilize for later use, the same below); culture temperature 25±2°C, humidity 20%-40%, light time 12 hours / day, light intensity 2500Lx , cultivated for 35 days.

[0028] 2. Strong seedling cultivation

[0029]Transf...

Embodiment 2

[0038] 1. Bud proliferation induction culture

[0039] Inoculate aseptic single buds of Chinese fir about 1.5-2.5cm long on the proliferation induction medium for proliferation induction, and transfer to fresh proliferation induction medium when 2-5 buds germinate at the base, and remove the original buds when transferring Bud tops to promote the growth of new shoots, thus forming clusters of secondary seedlings.

[0040] The formula of the described proliferation-inducing medium is that every liter contains: 0.5mg of 6BA, 0.05mg of NAA, 30g of white granulated sugar, 3.2g of agar powder and the rest of the MS medium to adjust the pH before sterilization The value is 5.85; the culture temperature is 25±2°C, the humidity is 20%-40%, the light time is 12 hours / day, the light intensity is 2500Lx, and the culture is 35 days.

[0041] 2. Strong seedling cultivation

[0042] Transfer the clustered secondary seedlings to the strong seedling medium for rejuvenation before rooting. T...

Embodiment 3

[0051] 1. Bud proliferation induction culture

[0052] Inoculate aseptic single buds of Chinese fir about 1.5-2.5cm long on the proliferation induction medium for proliferation induction, and transfer to fresh proliferation induction medium when 2-5 buds germinate at the base, and remove the original buds when transferring Bud tops to promote the growth of new shoots, thus forming clusters of secondary seedlings.

[0053] The formula of the described proliferation-inducing medium contains per liter: 3.0mg of 6BA, 30g of white granulated sugar, 3.5g of agar powder and the rest of the MS medium, adjusting the pH value before sterilization to 5.8; the culture temperature 25±2°C, humidity 20%-40%, light time 12 hours / day, light intensity 3000Lx, culture for 35 days.

[0054] 2. Strong seedling cultivation

[0055] Transfer the clustered secondary seedlings to the strong seedling medium for rejuvenation before rooting. The strong seedling cultivation is mainly to promote the grow...

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Abstract

The invention discloses a method for significantly improving the fir tissue culture production emergence rate. The method comprises the steps that 1, a fir single bud is grafted to a proliferation induction culture medium for proliferation induction, the top end of the original single bud is removed after cluster buds emerge, subculture is carried out, and cluster subculture plantlets are obtained; 2, the cluster subculture plantlets are grafted to a strong seedling culture medium for acclimatization and culture, and acclimatized cluster seedlings are obtained; 3, with single buds sheared off, the acclimatized cluster seedlings are grafted to a rooting induction culture medium for rooting induction to obtain rooting seedlings; 4, the rooting seedlings are transplanted to surface sandy yellow soil. By means of the method, the fir clone tissue culture seedling proliferation times can be 6-8, a single-bottle acclimatization subculture seedling can produce 50-70 rooting seedlings, the rooting rate reaches 100%, and the transplanting survival rate can reach more than 98%; the fir tissue culture production emergence rate can be significantly improved, the cost is saved, production benefits are improved, lateral bud differentiation is reduced after rooting seedlings are transplanted, and the apical dominance is obvious.

Description

technical field [0001] The invention belongs to the field of tissue culture of plant clones, and in particular relates to a method for significantly improving the emergence rate of Chinese fir tissue culture. Background technique [0002] Chinese fir (Cunninghamialanceolata) is the most important fast-growing and high-yielding tree species in southern my country. It has excellent characteristics such as fast-growing, high-yielding, good material properties, and wide application. After the disadvantages of eucalyptus became increasingly prominent, the planting amount of fast-growing fir is increasing day by day, which has the potential to catch up with fast-growing eucalyptus. Because Chinese fir is a cross-pollinated tree species, it is propagated by seeds, and the individual differentiation is large, so it is difficult to maintain the traits of the female parent of Chinese fir; while using conventional asexual reproduction methods such as cuttings, layering or grafting, the ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 唐黎明林武陈云峰吕金凤易平郭秀丽莫开敏
Owner 广西壮族自治区国有黄冕林场
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