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Compound for inhibiting growth of xanthomonas citri

A technology of citrus canker and a compound, which is applied in the field of bacteriostatic and bactericidal activity of citrus canker, and can solve the serious problems of rock sugar orange orchards and the like

Inactive Publication Date: 2015-12-16
许自协
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, most of the sweet orange orchards have also suffered from spring shoots, and the rock sugar orange orchards in some areas are sometimes very serious.

Method used

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  • Compound for inhibiting growth of xanthomonas citri
  • Compound for inhibiting growth of xanthomonas citri

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Measure the minimum inhibitory concentration MIC value.

[0015] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0016] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0017] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.

[0018] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially on...

Embodiment 2

[0025] Measure the minimum bactericidal concentration MBC value.

[0026] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0027] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0028] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.

[0029] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially ...

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Abstract

The invention discloses a compound which has bacteriostasis and bactericidal activity to xanthomonas citri. The minimal inhibitory concentration (MIC) value of the xanthomonas citri is 8 micrograms / mL, and the minimal bactericidal concentration (MBC) value of the xanthomonas citri is 32 micrograms / mL.

Description

technical field [0001] The invention relates to a new application of the compound, clarifies the relationship between the chemical structure of the medicine and the biological activity of bacteria, and more specifically explores the bacteriostatic and bactericidal activities of the compound on canker sores of citrus. Background technique [0002] Citrus is an important tropical and subtropical fruit tree, and its production is widely distributed. There are 145 countries producing citrus in the world, and its output ranks first in the world. According to FAO statistics, in 2011, the world's total output of W orange reached 129 million tons, accounting for 21.2% of the world's total fruit output. China is the world's largest producer of citrus. According to national agricultural statistics, in 2011, the cultivated area of ​​citrus reached 34.325 million mu, and the output reached 29.44 million tons. Citrus is widely cultivated in 19 mainland provinces (cities, districts) in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D413/04A01N43/86A01P3/00
CPCC07D413/04A01N43/86
Inventor 不公告发明人
Owner 许自协
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