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Biological protein two-dimensional nano-film prepared by using lysozyme and preparation method of biological protein two-dimensional nano-film

A two-dimensional nano and protein technology, applied in the direction of nanotechnology, can solve the problems of difficult realization of macroscopic organization and complex synthesis route, and achieve the effect of simple preparation method and environmental friendliness

Active Publication Date: 2015-12-16
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is more difficult to achieve large-scale (more than centimeter-scale) macroscopic tissues formed by self-assembly of microscopic building blocks, and complex synthetic routes are often involved to obtain target products or peptides

Method used

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  • Biological protein two-dimensional nano-film prepared by using lysozyme and preparation method of biological protein two-dimensional nano-film
  • Biological protein two-dimensional nano-film prepared by using lysozyme and preparation method of biological protein two-dimensional nano-film
  • Biological protein two-dimensional nano-film prepared by using lysozyme and preparation method of biological protein two-dimensional nano-film

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Add 0.1433g of tris(2-carboxyethyl)phosphine into 10mL of 10mmol / L tris(2-carboxyethyl) buffer solution with a pH value of 7.4, adjust the pH value to 5.0 with NaOH, and prepare 50mmol / L of tris(2-carboxyethyl) Tris buffer solution of phosphine; add 20mg lysozyme to 10mL10mmol / L tris buffer solution with pH value of 7.4, and prepare tris buffer solution of 2mg / mL lysozyme Buffer solution; mix 10mL of 50mmol / L tris(2-carboxyethyl)phosphine tris buffer solution with 10mL 2mg / mL lysozyme tris buffer solution, and let stand at room temperature for 50 minutes , forming a thin film on the surface of the mixture (such as figure 1 Shown), that is, biological protein two-dimensional nano film, its thickness is about 50nm. Depend on figure 2 and image 3 It can be seen that the two-dimensional nano-film of biological protein is formed by the self-assembly of nanoparticles with a particle size of about 30nm-50nm generated during the phase transition process.

Embodiment 2

[0027] Add 0.1147g of tris(2-carboxyethyl)phosphine into 10mL of 10mmol / L tris(2-carboxyethyl) buffer solution with a pH value of 7.4, adjust the pH value to 5.0 with NaOH, and prepare 40mmol / L of tris(2-carboxyethyl) base) Tris buffered solution of phosphine; 50mg lysozyme was added into 10mL10mmol / L pH value of 7.4 Tris buffered solution to prepare 5mg / mL Tris of lysozyme Buffer solution: Mix 10mL of 40mmol / L tris (2-carboxyethyl) phosphine tris buffer solution with 10mL 5mg / mL lysozyme tris buffer solution, and let stand at room temperature for 50 minutes , forming a thin film on the surface of the mixed solution, that is, a two-dimensional nano-film of biological protein, with a thickness of about 60nm.

Embodiment 3

[0029] Add 0.1433g of tris(2-carboxyethyl)phosphine into 10mL of 10mmol / L tris(2-carboxyethyl) buffer solution with a pH value of 7.4, adjust the pH value to 6.0 with NaOH, and prepare 50mmol / L of tris(2-carboxyethyl) Tris buffer solution of phosphine; add 20mg lysozyme to 10mL10mmol / L tris buffer solution with pH value of 7.4, and prepare tris buffer solution of 2mg / mL lysozyme Buffer solution; mix 10mL of 50mmol / L tris(2-carboxyethyl)phosphine tris buffer solution with 10mL 2mg / mL lysozyme tris buffer solution, and let stand at room temperature for 50 minutes , forming a thin film on the surface of the mixed solution, that is, a two-dimensional nano-film of biological protein, with a thickness of about 60nm.

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Abstract

The invention discloses a method for preparing a biological protein two-dimensional nano-film by using lysozyme. According to the method, nano-particles which are generated by phase transformation reaction of lysozyme molecules and tris (2-carboxyethyl) phosphine hydrochloride are self-assembled on a gas-liquid interface through surface and interface induction to form a biological protein two-dimensional nano-film, or a base material is in contact with the surface of solution in a preparation process of the film, and the nano-particles which are generated by phase transformation of the lysozyme are directly self-assembled on a liquid-solid surface through surface and interface induction to form the two-dimensional nano-film, namely, the nano-particles grow on the surface of the base material in an in-situ manner. The preparation method is simple and environment-friendly; the thickness of the prepared biological protein two-dimensional nano-film is about 30-80 nanometers, surface roughness is small, transparency is high, adhesivity is good, and the method can be used for modifying surfaces of base materials.

Description

technical field [0001] The invention belongs to the technical field of preparation of biological protein two-dimensional nano film, and in particular relates to a biological protein two-dimensional nano film prepared by using lysozyme and a preparation method thereof. Background technique [0002] Among the various physiological functions in nature, the self-assembly of protein aggregates is particularly common. For example: amelogenin can self-assemble to form nanospheres, and these nanosphere systems assemble to form a ribbon structure. This self-assembled structure plays a key template role by controlling the growth of hydroxyapatite crystals to achieve Biomineralization of tooth enamel. Nowadays, protein two-dimensional nanofilms formed by self-assembly have attracted extensive attention from the scientific community. Especially during the phase transition, supramolecular aggregates are formed by the spontaneous aggregation of hydrophobic particles, thereby forming a t...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L89/00B82Y40/00
Inventor 杨鹏哈媛王德辉
Owner SHAANXI NORMAL UNIV
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