Primers and method for identifying takifugu obscurus fry and takifugu xanthopterus fry

Abstract

The invention relates to primers and a method for identifying takifugu obscurus fry and takifugu xanthopterus fry. The pair of primers is named as F-FXF147, and the sequences of the primers are shown as SEQ ID No.1 and SEQ ID No.2. In the verification process, a common DNA extraction reagent kit is used for extracting the genomic DNA of the takifugu obscurus fry and the genomic DNA of the takifugu xanthopterus fry, a DNA sample of takifugu obscurus and a DNA sample of takifugu xanthopterus are subjected to PCR amplification through the primers, PCR products are detected through 2.2% sepharose gel (EB dyeing) electrophoresis, and the fry with strips around 300 bp are the takifugu obscurus, and the fry with strips around 600 bp are the takifugu xanthopterus. According to the primers and the method for identifying the takifugu obscurus fry and the takifugu xanthopterus fry, verification can be conducted on the basis that only a small number of tail fins are sheared instead of killing the fry, and the primers and the method have the advantages of being high in stability, good in repeatability, high in practicability, easy to operate, capable of quickly achieving verification and the like.

Description

technical field [0001] The invention belongs to the field of aquaculture, and relates to a molecular biological technology identification method for fry of the same genus. More specifically, the invention relates to accurately identifying fry of puffer puffer obscurus and puffer striped by using microsatellite markers. Background technique [0002] Fugu (Takifugufasciatus) belongs to Letrodontiforms, Tetraodontidae, and Fugu. The middle and lower reaches of the Yangtze River is an aquatic product with high economic value. It ranks first among the "Four Famous Fishes" of the Yangtze River and is a model of traditional cuisine. [0003] Striped puffer (Takifuguxanthopterus) belongs to Letrodontiforms (Letrodontiforms), Tetraodontidae (Tetraodontidae), Fugu (Fugu), commonly known as yellow fin puffer, flower boat, etc., is also a migratory fish, and its The habitat and breeding season of oriental puffer are similar. It is mainly distributed in the East China Sea, South China S...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for quickly and accurately identifying puffer puffer and striped puffer fry, to overcome the existing defects that the morphological identification is highly subjective and cannot effectively identify the two fry, and the present invention On the basis of not killing the fry, only a small amount of caudal fin or other fish tissue parts can be cut off for identification. It has the advantages of high stability, simple operation, good repeatability, strong practicability, and rapid and accurate identification.

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