Indoor rice variety Magnaporthe oryzae resistance identification method

A technology for resistance identification and rice blast, applied in the agricultural field, can solve problems such as loss of resistance of disease-resistant varieties, and achieve the effect of saving labor and time.

Active Publication Date: 2015-12-30
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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Problems solved by technology

The use of disease-resistant varieties to resist the prevalence of rice blast is an effective safety measure, but in actual production, the resistance of a single disease-resistant variety will be lost after long-term large-scale planting, resulting in the prevalence and outbreak of rice blast. It is a long-term scientific research task for rice breeding and disease resistance researchers to constantly search for new resistance source materials

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Embodiment Construction

[0017] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0018] Isolation of single spore and preparation of spore suspension and inoculation method

[0019] Spray inoculation, spot inoculation, and injection inoculation are the three most widely used inoculation methods for rice blast. Through years of experiments, our research team combined the above three methods for indoor identification of rice blast resistance.

[0020] Use the spore shake-off method to separate monospores of Magnaporthe grisea: first soak the panicle blast specimens in sterile water for 48 hours, and then move them to a petri dish for 48 hours of moisturizing culture to allow them to sporulate. Water-absorbing paper, one end of the absorbent paper is made into a paper pillow, so that both ends of the specimen are in contact with the absorbent paper, and the lesion is suspended (temperature 26°C, light:dark=12h:12h). Wh...

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Abstract

The invention discloses an indoor rice variety Magnaporthe oryzae resistance identification method, comprising the steps of firstly performing spray inoculation indoors, recording susceptible varieties in a classifying way, and ending a test under the situation of susceptible contrasting with LTH; growing seedlings again for nosotropic varieties, then performing indoor in vitro leaf segment liquid dripping inoculation, recording susceptible varieties in the classifying way, and ending a test; growing seedlings again for nosotropic varieties, then performing liquid dripping inoculation, and timely recording morbidity conditions after a disease state is stable. The technology set of resistance screening, combining three inoculation methods, avoids the defect of a single inoculation method and saves manpower and material resources, finally the indoor Magnaporthe oryzae-resisting capability of a rice variety can be really shown, and test results can lay a stable and reliable foundation for the excavating of field popularized varieties and variety resistance gene, and the like.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for identifying blast resistance of indoor rice varieties. Background technique [0002] Rice is an important food crop in the world, especially in Asia, and rice blast (Magnaportheoryzae) caused by Ascomycetes is one of the main diseases that affect the safe production of rice. In addition, the actual control of rice blast is complicated and widely distributed. As well as the severity of the damage, rice blast has always been highly concerned by governments and researchers in various places. [0003] Hubei Province is located in the middle and lower reaches of the Yangtze River, with complex landforms, including mountains, hills and flat lakes. Rice has always been the crop with the largest planting area and total output in Hubei Province. The sown area accounts for more than 50% of the province's total grain sown area, the total output accounts for abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01G7/06
CPCA01G7/00A01G7/06
Inventor 杨小林张舒吕亮常向前袁斌
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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