Cell resuscitator

A resuscitator and cell technology, applied in the field of cell biology, can solve the problems of low degree of automation, difficult to accurately control cell recovery time and temperature, etc., to achieve dry and wet separation, improve quality and stability, and improve work efficiency. Effect

Active Publication Date: 2015-12-30
SHANGHAI ORIGINCELL BIOLOGICAL CRYO EQUIP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a cell resuscitator in order to overcome the shortcomings of the prior art that cell resuscitation requires manual hand-held cell tubes, the degree of automation is not high, and the time and temperature of cell resuscitation are difficult to accurately control due to human factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] figure 1 A cell resuscitator is shown for rapidly reviving cells stored cold in liquid nitrogen. The present invention comprises a water bath 1, a lifting mechanism 2, a carrying device detachably arranged on the lifting mechanism 2, a controller 4 and a distance sensor (not shown in the figure).

[0030] In this embodiment, the lifting mechanism 2 is a screw lifter, including a motor (not shown in the figure), a screw rod 21 , and a slider mechanism 22 . The controller 4 is electrically connected with the lifting mechanism 2, and is used for controlling the movement of the lifting mechanism 2 and putting the carrying device into the water bath 1. The carrying device is a tray 3 , but is not limited to the tray 3 , and may be a clamp that can hold the cryopreservation tube 5 . Positioning holes are provided on the tray 3, and the tray 3 can be used to fix cryopreservation tubes 5 of different sizes in batches.

[0031] In this embodiment, the cell resuscitator furthe...

Embodiment 2

[0037] Embodiment 2 is substantially the same as Embodiment 1, as Figure 4 As shown, the difference is that the cell resuscitator also includes a first isolation sleeve 6 and a cover plate 7, the first isolation sleeve 6 is used to isolate the carrying device and the water bath 1, and the cover plate 7 is arranged on the lifting mechanism 2 and located on the carrying device The upper cover plate 7 matches the opening of the first isolation sleeve 6 , and the cover plate 7 and the first isolation sleeve 6 can form a sealed space. When performing cell recovery, the lifting mechanism 2 places the tray 3 with several cryopreservation tubes 5 in the first isolation sleeve 6, avoiding the direct contact between the cryopreservation tubes 5 and water, and when the lifting mechanism 2 moves to the lowest position, The cover plate 7 just covers the opening of the first isolation sleeve 6 and seals the cryopreservation tube 5 in a sealed environment, reducing the influence of external...

Embodiment 3

[0039] Embodiment 3 is substantially the same as Embodiment 1, as Figure 5 As shown, the difference is that the carrying device includes a plurality of second isolation sleeves 8, and the second isolation sleeves 8 are detachably arranged on the carrying device. The second isolation sleeve 8 is used to carry the cryopreservation tube 5 and to isolate the cryopreservation tube 5 from the water bath 1, avoiding the direct contact between the cryopreservation tube 5 and water, and realizing the dry-wet separation of the cryopreservation tube 5 and the water bath 1. The second spacer 8 is made of a material with excellent thermal conductivity, and its specifications are specially designed according to the specifications of the cryotubes 5. Each cryotube 5 corresponds to a spacer, so that it can take into account better heat conduction. In the experiment, the cryopreservation tube 5 was directly placed in the second isolation sleeve 8, and the controller 4 received the distance si...

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PUM

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Abstract

The invention discloses a cell resuscitator. The cell resuscitator comprises a water bath kettle, a lifting mechanism, a carrying device and a controller, wherein the carrying device is arranged on the lifting mechanism; the controller is electrically connected with the lifting mechanism and is used for controlling the movement of the lifting mechanism and putting the carrying device into the water bath kettle. According to the cell resuscitator disclosed by the invention, by the combination of the lifting mechanism and the controller, a process of automatically resuscitating cells in large batches is achieved, the time and temperature of resuscitation can be accurately controlled, and the process parameters of resuscitation are recorded; moreover, the possible pollution on frozen cells caused by water in the water bath kettle is avoided, the resuscitation quality of the cells is greatly improved, the burden on an operator is alleviated, and the working efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a cell resuscitator. Background technique [0002] The main experimental objects of cell biology research are various primary cultured or subcultured cells or cell lines. In order to avoid the pollution caused by the cultured cells in the long-term in vitro culture process or the gene mutation caused by multiple passages, the experimenter will freeze the cultured cells during the vigorous growth period, and store the frozen cells in a container with cell freezing solution The cells are frozen in tubes, and then placed in liquid nitrogen at -196°C. In this ultra-low temperature environment, the cells can be stored for a long time. When the experiment is needed, the experimenter can revive the frozen cells at 37°C for the required experiment, which can ensure the genetic stability of the cells to the greatest extent. [0003] Cell recovery is the process of taking frozen cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34
CPCC12M41/12C12M41/48
Inventor 何晓文陈小功张震徐艳红葛松陆婷赵鸿莲瞿建国
Owner SHANGHAI ORIGINCELL BIOLOGICAL CRYO EQUIP CO LTD
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