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A microfluidic chip for cell printing

A microfluidic chip and cell printing technology, applied in the field of cell analysis, can solve the problem of not being able to print a multi-layer structure, achieve the effect of no cleaning, strong scalability, improve capture efficiency, and achieve accurate capture

Active Publication Date: 2017-04-05
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its capture success rate is only 90% to 94%, and its capture time is usually about half an hour, and it cannot print multi-layer structures like inkjet printing

Method used

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  • A microfluidic chip for cell printing
  • A microfluidic chip for cell printing
  • A microfluidic chip for cell printing

Examples

Experimental program
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Effect test

example

[0045] The microfluidic chip of the present invention is used to print cancer cells with an average diameter of about 10 microns: the preparation of such as figure 2 In the microfluidic chip shown, the microchannel 3 is engraved on the lower substrate 1, the materials of the upper cover 2 and the lower substrate 1 are both PDMS, the upper substrate 1 and the lower substrate 2 are bonded together, and the depth of the microchannel 3 is The width is 30 microns and the width is 35 microns. The distance between the hook-shaped micro-baffle structure 8 and the bottom of the micro-flow channel 3 is 8 microns, and the gap between the hook-shaped micro-baffle structure 8 and the upper cover plate 2 is 22 microns. In the flow blocking structure 8, the bending angle is a right angle. Such as Figure 5a As shown, the width of each straight side in the hook-shaped micro-blocking structure 8 is 5 microns, the distance d2 between the hook-shaped micro-blocking structure and the wall surfa...

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Abstract

The invention discloses a microfluidic chip for cell printing, wherein a micro-channel comprises an arc-shaped main channel which is located among a cell suspension inlet, a culture solution inlet and a solution outlet; the main channel, in the middle position, is communicated with a compressed air channel and a jet outlet, so that a cell catching region is formed; a hook-shaped micro-flow-baffle structure for catching cells is arranged in the cell catching region; the concave side of the hook-shaped micro-flow-baffle structure faces to the incoming direction of the main channel and the jet outlet; the maximum distance between the hook-shaped micro-flow-baffle structure and the near-end wall of the main channel is more than the diameter of a target cell but is less than twice of the diameter of the target cell, while the minimum distance is less than the diameter of the target cell; and the minimum distance between the hook-shaped micro-flow-baffle structure and the far-end wall of the main channel is more than the diameter of the target cell.

Description

technical field [0001] The invention relates to cell analysis technology, in particular to a microfluidic chip for cell printing. Background technique [0002] Single-cell analysis has developed into a key issue in the study of cell function in the field of cell biology, and limited tools and technical support have made the isolation of single cells and subsequent single-cell research more and more prominent. Sorting single cells at a fixed location is beneficial for maintaining the state of the cells over a longer period of time. There are two modes of contact and non-contact in the technology of sorting cells. Sorting cells is often combined with microfluidic systems, which provide measurement and continuous power supply of nutrients or reagents on very short time scales, and occasionally provide "Sorting release" function. Single-cell manipulation on microfluidic chips is a method of driving cells within micron-scale channels and transporting them to predetermined sites...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00B01L3/00B41J29/393
CPCB01L3/5027B41J29/393C12M33/00
Inventor 张旻雒文伯
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV