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In vitro culture system kit for raising human sperm motility and application thereof

A technology for in vitro culture and sperm motility, applied in the field of XX, can solve the problem of insignificant improvement of sperm motility, and achieve the effects of improving forward motility, improving pregnancy outcome, and improving fertilization rate.

Active Publication Date: 2016-01-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned culture medium mainly provides a buffer and a basic energy supply environment for sperm, and has no obvious effect on improving sperm motility

Method used

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  • In vitro culture system kit for raising human sperm motility and application thereof
  • In vitro culture system kit for raising human sperm motility and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Sample source: Three normal motility sperm specimens (standard according to the fifth edition of WHO)

[0024] Sperm processing:

[0025] 1. Preheat 10 mL of mHTF with a volume fraction of 10% SSS (as a pre-culture control group), 2 mL of LIsolate sperm separation solution and 10 mL of the in vitro culture solution of the present invention.

[0026] 2. Place the semen collection cup with the semen in a 37°C water bath and take it out after liquefaction.

[0027] 3. Mix the preheated mHTF containing 10% SSS with the liquefied semen, slowly add it to the Isolate sperm separation solution, and centrifuge at 300g for 10 minutes.

[0028] 4. After centrifugation, the supernatant was discarded, the lower layer was separated and the sperm was examined under a microscope, and the sperm motility was recorded as the control group before culture.

[0029] 5. Mix the remaining sperm of step 4 with the sperm in vitro culture solution of the present invention at a concentration of 10 million / ml...

Embodiment 2

[0033] Sample source: three sperm specimens of asthenospermia patients (standard according to the fifth edition of WHO)

[0034] Sperm processing:

[0035] 1. Preheat 10 mL of mHTF with a volume fraction of 10% SSS (as a pre-culture control group), 2 mL of LIsolate sperm separation solution and 10 mL of the in vitro culture solution of the present invention.

[0036] 2. Place the semen collection cup with the semen in a 37°C water bath and take it out after liquefaction.

[0037] 3. Mix the preheated mHTF containing 10% SSS with the liquefied semen, slowly add it to the Isolate separation solution, and centrifuge at 300g for 10 minutes.

[0038] 4. After centrifugation, the supernatant was discarded, the lower layer was separated and the sperm was examined under a microscope, and the sperm motility was recorded as the control group before culture.

[0039] 5. Mix the remaining sperm of step 4 with the sperm culture system of the present invention at a concentration of 10 million / ml, and ...

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Abstract

The invention discloses an in vitro culture system kit for raising human sperm motility and an application thereof and belongs to the field of XX technology. The kit comprises 9mL of mHTF, 25 microliters of Hexadecanoic Acid, 25 microliters of Trans-13-Octadecenoic Acid, 20 microliters of 9,12-Octadecenoic, 30 microliters of Arachidonic Acid and 50 microliters of Docosahexaenoate Acid. The reagents in the kit are mixed and balanced at 37 DEG C for 2-3 h so as to obtain 10.15 ml of a sperm culture system; and sperm with the concentration of 10 millon / mL that is separated by a sperm split liquid is resuspended by the use of the above sperm culture system, blending is lightly carried out, and the product is put in a thermotank of 37 DEG C for 90-120 min, so as to achieve the effect of raising sperm motility. By the use of the kit, sperm progressive motility can be remarkably raised. When the kit is used in the technologies of artificial insemination and in vitro fertilization, pregnancy outcome can be improved, and rate of fertilization can be raised. Meanwhile, a new method is provided for treatment of patients with severe asthenospermia.

Description

Technical field [0001] The invention relates to an in vitro culture system kit for improving human sperm motility and its application, and belongs to the XX technical field. Background technique [0002] According to data from the World Health Organization, about 15% of couples of childbearing age are facing the threat of infertility. my country's recent survey results show that domestic infertility accounts for 10% of married couples, and the incidence is on the rise. Among them, about half of human infertility patients are male infertility patients, and asthenospermia is one of the common causes of male infertility. Clinically, artificial insemination is often used for treatment. Data shows that better sperm motility is an important condition for improving the success rate of assisted reproductive technology. [0003] The main components of semen are sperm and seminal plasma. Sperm originates from the testis and is the main body of fertilization. Its plasma membrane contains a v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
Inventor 王英男杨勇吴亦波毛健
Owner JIANGNAN UNIV
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