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Application of emulsified perfluorocarbon liquid in preparation of lung preserving fluid

A technology of organ preservation solution and fluorocarbon, which is applied in the medical field, can solve the problems of poor effect, and achieve the effects of increasing perfusion flow, reducing blood viscosity, and rapid gas exchange

Inactive Publication Date: 2016-01-20
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, some studies have found that LPD solution is less effective in anti-inflammation, reducing the exudation and immersion of inflammatory cells during ischemia-reperfusion injury of the donor lung. Long Xiaomao stored the donor lung with LPD solution perfused through the pulmonary artery and vein at 4°C. In low-temperature LPD solution, it was found that 80% to 90% of the lung tissue had a large number of inflammatory cells in the alveolar cavity

Method used

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  • Application of emulsified perfluorocarbon liquid in preparation of lung preserving fluid
  • Application of emulsified perfluorocarbon liquid in preparation of lung preserving fluid
  • Application of emulsified perfluorocarbon liquid in preparation of lung preserving fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Changes in hemodynamics and pathological scores when two PFC administration methods are used to treat ALI

[0053] 24 adult healthy domestic pigs (purchased from the Guangdong Institute of Animals), with an average body weight of 25 ± 5 kg; the animals were intramuscularly injected with ketamine 10 mg kg -1 After opening the marginal ear vein, inject 0.02 mg·kg intravenously -1 And ketamine 2mg·kg -1 After a supine tracheotomy, a No. 7.0 tracheal tube was placed, and mechanically controlled ventilation (740 ventilator, the United States) was performed. The respiratory parameters were inspired oxygen volume fraction (FiO 2 ) is 1.0, tidal volume (TV) is 12mL·kg -1 , the respiratory rate (respiratory rate, RR) is 40 times min, the inspiration-expiration ratio (I:E) is 1:2, and the positive end-expiratory pressure (PEEP) is 4cmH 2 O. During the experiment, the animals were continuously infused with sugar-salt mixture (mass fraction: 0.45% sodium chloride, mas...

Embodiment 2

[0068] Example 2 Effects of emulsified fluorocarbon solution (FCE), UW solution and their mixture on anti-inflammatory and antioxidant capacity and pathological ultrastructure of isolated rat lungs during different storage periods

[0069] The establishment of the isolated lung perfusion model refers to the method described by Fischer, S to establish the isolated lung perfusion model:

[0070] (1) Rats (purchased from Guangdong Medical Experimental Animal Center) were anesthetized: intraperitoneal injection of 50 mg / kg pentobarbital sodium with a mass fraction of 1%, intramuscular injection of atropine sulfate 0.4 mg / kg on the outside of the hind limb of the rat 30 minutes before the operation ; After the rats were successfully anesthetized, the supine position was fixed, and the skin of the chest, abdomen and neck was prepared;

[0071] (2) Artificial ventilation: cut longitudinally the skin, muscular layer and fascia of the neck, and a relatively large thyroid gland can be s...

Embodiment 3

[0079] Example 3 Protective effect of emulsified fluorocarbon solution combined with adenosine A2a receptor agonist on donor lung.

[0080] The experimental rats (purchased from Guangdong Provincial Medical Experimental Animal Center) were adaptively fed for one week, and they had free access to food and water during this period. Then the rats were randomly divided into four groups: emulsified fluorocarbon solution group (FCE), low-potassium dextran group (LPD), emulsified fluorocarbon solution+A2a group (FCEA) and low-potassium dextran+A2a group (LPDA), 12 in each group. Only. Among them, the lung perfusion fluid and preservation fluid of the FCE group and the LPD group were FCE fluid and LPD fluid respectively; / kg) mixed solution; establishment of isolated lung perfusion model (with reference to Fischer, the method described in S establishes isolated lung perfusion model):

[0081] (1) Rat anesthesia: intraperitoneal injection of 50 mg / kg pentobarbital sodium with a mass ...

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Abstract

The invention belongs to the field of medicine, and particularly relates to an application of emulsified perfluorocarbon liquid in preparation of a lung preserving fluid. The emulsified perfluorocarbon liquid has the characteristics of high oxygen carrying capability and high oxygen releasing speed; blood visible components are not contained, so that cytotoxicity and generation of oxygen free radicals are reduced; a pH value is between 7.4 and 7.6, so that cellular acidification can be prevented, and cellular damages caused by activation of protease and phosphatase in cold preservation are inhibited; the colloid osmotic pressure is 390 mmH2O, so that cellular edema can be prevented; particles are small, the oxygen carrying capability is high, the gas exchange speed is fast, the emulsified perfluorocarbon liquid can easily pass through tiny capillaries and partially blocked pulmonary arterioles, the establishment of collateral circulation is promoted, the filling and oxygen supply effects are achieved, and oxidative damages are reduced; the emulsified perfluorocarbon liquid is an inert compound and can form dense and diffused physical barriers on the surfaces of cell membranes, so that the contact of inflammatory factors with target cells is prevented. The emulsified perfluorocarbon liquid is suitable for filling and preserving important transplanting organs such as lungs and the like.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of an emulsified fluorocarbon liquid in the preparation of a lung organ preservation liquid. Background technique [0002] In the 21st century, medical research has entered the era of organ transplantation. Various organ transplants have brought new ideas and hopes for the clinical treatment of difficult diseases and chronic organ failure. Lung transplantation is a life-saving treatment for patients with end-stage lung diseases. The development and improvement of this treatment requires the use of various animal models. Without this process, clinical lung transplantation will not become a reality and possible. The transplanted lung preservation solution has been found through a large number of basic clinical studies, and reasonable vascular perfusion and isolated lung preservation play a key role in the success of lung transplantation. Effective preservation o...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 彭雪梅帅兵刘慧慧席露谢娟华刘嘉羿邹启荣卢春英易艳萍
Owner JINAN UNIVERSITY
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