Intervention effect of azoxystrobin on oral cancer and precancerous lesions
A technology for azoxystrobin and oral cancer, which is applied in the field of intervention of azoxystrobin for oral cancer and precancerous lesions, and can solve the problems of chemotherapy failure, poor treatment effect, and no correlation between azoxystrobin and cancer.
Active Publication Date: 2018-05-18
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology
At present, oral cancer is mainly treated with a comprehensive sequence of surgery, radiotherapy, and chemotherapy, but the treatment effect is often poor, and the 5-year survival rate is less than 50%.
[0004] Defects in prior art background: Chemotherapy is one of the commonly used methods in the clinical treatment of oral cancer, and it plays an important role in the treatment of oral cancer. However, in some cases, the body develops drug resistance to chemotherapeutic drugs after chemotherapy, resulting in chemotherapy failure and side effects. bigger
So far, no research on the correlation between azoxystrobin and cancer has been found at home and abroad
Method used
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[0021] SCC15 cells were cultured in DMEM-F12 medium, CAL27 cells and DOK cells were cultured in DMEM high-glucose medium, 10% Gibco fetal bovine serum, 100U / ml penicillin, 100U / ml streptomycin, and placed in 5% CO 2 , 95% air, and 37°C saturated humidity incubator. The cells grow on the wall and are subcultured every 2-3 days. The cells required for the experiment are in the logarithmic growth phase.
[0022] Cell proliferation was detected by CCK-8 method. Experimental settings: In the experimental group, the concentrations of azoxystrobin were 1.5 μg / ml, 3 μg / ml, 6 μg / ml, and 12 μg / ml; in the solvent control group, the same concentration of DMSO was used as in the experimental group. The cells in the logarithmic growth phase were digested with trypsin containing 0.25% EDTA, centrifuged, and the supernatant was discarded. Adjust the cell concentration to 2.5 × 10 with medium containing 10% serum 4 pieces / ml. Inoculate 200 μl of cell suspension into each well of a 96-well ...
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The invention relates to the novel application of a compound azoxystrobin (methyl (E)-2-{2-[6-(2-cyanophenoxy) pyrimidine-4-yloxy] phenyl}-3-methoxyacrylate) and a preparation used for remarkably inhibiting oral cavity cancer cells and belongs to the field of research and development of antineoplastic drugs. Azoxystrobin is simple and convenient in obtaining method and definite in chemical construction, a CCK-8 method is adopted for detecting proliferative activity of the oral cavity cancer cells SCC15 and CAL27 and proliferative activity of oral cavity precancerous lesion DOK cells, experimental results show that the obvious anti-proliferative effect is achieved, the intervention effect of azoxystrobin for oral cavity cancer and precancerous lesions provides an experimental basis for research and development of new drugs for clinically treating oral cavity cancer, and azoxystrobin can be applied to preparations for inhibiting growth of the oral cavity cancer cells.
Description
technical field [0001] The present invention relates to a new application of a compound, in particular to a new compound interfering with the growth of oral cancer cells. Background technique [0002] Oral cancer is a common malignant tumor in the head and neck, accounting for about 3% of systemic malignant tumors, ranking sixth. The incidence of oral cancer is on the rise worldwide, and the age of onset is getting younger and women are increasing. It is estimated that there are 274,000 new cases worldwide every year. The oral cavity is an important anatomical part of the human body. The main symptoms of oral cancer patients are damaged appearance, difficulty in eating and pain. In the advanced stage, there are also a series of clinical manifestations such as difficulty in opening the mouth, decreased saliva, and stiff neck. At present, oral cancer is mainly treated with a comprehensive sequence of surgery, radiotherapy, and chemotherapy, but the treatment effect is often p...
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IPC IPC(8): A61K31/505A61P35/00C07D239/52
Inventor 汤晓飞王春晓陈慧张敏
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV