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Microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method

A technology of functional microcapsules and fluorescent tracers, applied in the field of biomedical materials, can solve the problems of fluorescent agent application limitations, fluorescence failure, long excitation time, etc., to ensure fluorescent tracer performance, a wide range of material sources, and rapid preparation methods easy effect

Inactive Publication Date: 2016-01-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of biomedical research, fluorescent labels are commonly used to calibrate research objects, but the fluorescence will be invalidated due to too long excitation time, which limits the application of general fluorescent agents in biomedicine
However, there is no report on the preparation of microcapsules containing graphene quantum dots by ultrasonic method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The preparation method of microcapsules with fluorescence tracing and reduction-responsive drug release functions includes the following steps:

[0029] a. Disperse 0.1-100mg of graphene oxide quantum dots in 5-200ml of water ultrasonically;

[0030] b, in N 2 Under protection, inject 1-20ml of organic polymer or biopolymer containing sulfhydryl and amino groups at a concentration of 10mg / ml and 1-(3-dimethylaminopropyl)-3-ethyl carbon with a concentration of 10mg / ml Mixed solution of diimine hydrochloride;

[0031] c. Stir and react for 1-24 hours under anaerobic conditions, centrifuge and wash 3-5 times to remove unreacted organic polymers or biopolymers containing sulfhydryl and amino groups at the same time to obtain sulfhydryl graphene quantum dots;

[0032] d. Disperse the prepared thiolated graphene quantum dots uniformly in deionized water, use a test tube to measure the aqueous solution of thiolated graphene quantum dots and the oil phase containing hydrophobic drugs i...

Embodiment 1

[0039] Disperse 2mg of graphene oxide quantum dots in 20ml of water ultrasonically; in N 2 Under protection, inject 5ml of amino-polyethylene glycol-sulfhydryl with a concentration of 10mg / ml and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride with a concentration of 10mg / ml Mixed solution; stirred and reacted for 12 hours under anaerobic conditions, and centrifuged for 3 times to remove unreacted amino-polyethylene glycol-sulfhydryl groups to obtain sulfhydryl graphene quantum dots; 1mg sulfhydryl graphene quantum dots were uniformly dispersed in 10ml Dissolve 10 mg lomustine in 10 ml methyl silicone oil in deionized water; use a test tube to measure the aqueous solution of thiolated graphene quantum dots and the methyl silicone oil containing lomustine in a volume ratio of 5:1, and place them in In an ice-water bath; place the ultrasonic probe at the oil / water two-phase interface, irradiate ultrasonically for 5 minutes at a power of 400W; centrifuge the reaction so...

Embodiment 2

[0041] Disperse 2mg of graphene oxide quantum dots in 20ml of water ultrasonically; in N 2 Under protection, inject 5ml of amino-polyglutamic acid-sulfhydryl with a concentration of 10mg / ml and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride with a concentration of 10mg / ml Mixed solution; stirred for 16 hours under anaerobic conditions, 4 centrifugal washings to remove unreacted amino-polyglutamic acid-sulfhydryl groups to obtain sulfhydryl graphene quantum dots; 1mg sulfhydryl graphene quantum dots were uniformly dispersed in 10ml Dissolve 10 mg of paclitaxel in 10 ml of hydroxy silicone oil in deionized water; use a test tube to measure the aqueous solution of thiolated graphene quantum dots and the hydroxy silicone oil containing paclitaxel in a volume ratio of 5:1, and place them in an ice water bath; place the ultrasound probe At the oil / water two-phase interface, under 500W power, ultrasonic irradiation for 8min; the reaction solution was centrifuged and washed...

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Abstract

The invention relates to a microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method. A drug-loading microcapsule using a cross-linking film containing graphene quantum dots as a capsule wall and an oil phase loading a hydrophobic drug as a core is formed by: performing ultrasonic radiation on an aqueous solution obtained by modification with an organic polymer or biopolymer containing both sulfhydryl groups and amino groups and on the oil phase loading the hydrophobic drug. The graphene quantum dots are evenly distributed on the capsule wall of the microcapsule and are modified with the organic polymer or biopolymer, spacing among the graphene quantum dots is enlarged, and fluorescent tracing performance of the microcapsule during drug delivery is guaranteed; the formed capsule wall containing the graphene quantum dots encapsulates and protects a drug; drug release is effectively controlled by using reduction responsiveness of disulfide bonds; the preparation method is quick and simple and efficient and environment-friendly, and products are pure and nontoxic.

Description

Technical field: [0001] The invention relates to a biomedical material, in particular to a microcapsule with fluorescent tracer and reduction responsive drug release function containing graphene quantum dots on the capsule wall and a preparation method thereof. Background technique: [0002] Microcapsule is a kind of miniature container or package with a membrane-like wall shell. Due to its special structure and variability in performance, it has a wide range of applications in the fields of biomedicine, food and agriculture. There are various materials that can be used for the preparation of microcapsules, mainly including natural polymer materials, synthetic polymer materials and inorganic compounds. There are many methods for preparing microcapsules. According to the encapsulation mechanism, they can be roughly divided into three categories: physical methods, physical chemical methods, and chemical methods. With the development of scientific research, some new microencapsulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K9/50A61K31/337A61K31/4745A61K31/357A61K31/175
Inventor 崔学军董林林施超王洪艳
Owner JILIN UNIV
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