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Preparation method of magnetotactic bacteria model

A technology of magnetotactic bacteria and models, applied in bacteria and other directions, can solve the problems of no structure and simulation, and achieve the effect of complete structure

Active Publication Date: 2016-02-03
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the problem that the existing polymer-wrapped magnetic nanoparticles to establish a bacterial model only simulates the function but not the structure, and provides a method for preparing a magnetotactic bacterial model

Method used

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  • Preparation method of magnetotactic bacteria model

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specific Embodiment approach 1

[0012] Specific embodiment one: the preparation method of a kind of magnetotactic bacterium model described in the present embodiment is specifically carried out according to the following steps:

[0013] 1. Preparation of phospholipid vesicles: add dimyristoylphosphatidylcholine and cholesterol to chloroform to obtain a phospholipid solution, and coat the surfaces of two ITO glass electrodes with 5 μL to 7 μL of phospholipid solution respectively, and wait for the chloroform to evaporate to dryness to obtain Two pieces of ITO glass electrodes whose surfaces are covered with phospholipid solution; placing a polytetrafluoroethylene rectangular frame between two pieces of ITO glass electrodes whose surfaces are covered with phospholipid solution will obtain an electroforming device, and use vacuum grease to seal the electroforming device, and The polytetrafluoroethylene rectangular frame and two ITO glass electrodes covered with phospholipid solution form a middle cavity, and the...

specific Embodiment approach 2

[0018] Embodiment 2: This embodiment differs from Embodiment 1 in that the magnetic nanoparticles described in step 1 are ferroferric oxide magnetic nanoparticles with a particle diameter of 100 nanometers. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0019] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: in step 1, the AC voltage is set to 5V, the waveform is a sine wave, the frequency is set to 10 Hz, and the time control is 4 hours. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a preparation method of a bacteria model, in particular to a preparation method of a magnetotactic bacteria model. The preparation method provided by the invention solves the problem that for the process that polymers wrap magnetic nanoparticles to build a model, the simulation is only performed in a function and not performed in a structure in the prior art. The preparation method comprises the steps of 1, preparation of phospholipid vesicle; 2, preparation of the bacteria model, namely the preparation method of completing the magnetotactic bacteria model. The invention is used for the preparation of the magnetotactic bacteria model.

Description

technical field [0001] The invention relates to a preparation method of a bacterial model. Background technique [0002] As the prokaryote with the widest distribution and the largest number of individuals in nature, bacteria are the main participants in the material cycle of nature. Flagella are the movement organs of bacteria. Bacteria can change the movement state by adjusting the direction of flagella rotation (clockwise and counterclockwise). Establishing a bacterial model for in-depth research plays a crucial role in exploring the origin of life. At present, some literatures have reported the use of polymer-wrapped magnetic nanoparticles to establish bacterial models for research. This kind of simulation is only studied from the function, not from the structure. Contents of the invention [0003] The invention solves the problem that the existing polymer-wrapped magnetic nanoparticles to establish a bacterial model only simulates the function but not the structure, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 韩晓军宗薇
Owner HARBIN INST OF TECH
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