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Precise inactivation method and inactivated vaccine for maintaining the immune protection of Edwardella antigens

An antigen immunization and inactivated vaccine technology, applied in bacterial antigen components, methods of using microorganisms, antibacterial drugs, etc., can solve the problem of not finding the inactivation conditions for the immune protection of Edwardella antigens, and achieve the maintenance of immune protection. Effect

Active Publication Date: 2017-10-24
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] After our research, we found that the previous failures in the study of Edwardsiella inactivated vaccines were due to the failure to find inactivation conditions that can effectively maintain the immune protection of Edwardsiella antigens

Method used

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  • Precise inactivation method and inactivated vaccine for maintaining the immune protection of Edwardella antigens
  • Precise inactivation method and inactivated vaccine for maintaining the immune protection of Edwardella antigens
  • Precise inactivation method and inactivated vaccine for maintaining the immune protection of Edwardella antigens

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Embodiment 1

[0022] (1) Establishment of formalin as an inactivating agent for precise inactivation of Edwardsiella

[0023] ①Cultivation of Edwardsiella tarda

[0024] Edwardsiella tarda can be cultured in LB medium (tryptone 10g, yeast extract 5g, NaCl 10g, deionized water to 1L, pH 7.0) or tryptone soybean broth (TSB, tryptone 17g, plant peptone 3g , glucose 2.5g, NaCl 5.0g, K 2 HPO 4 2.5g, deionized water to 1L, pH7.3) or 2216E medium (according to the presence or absence of seawater in the medium composition, it can be divided into two types, one: yeast extract 1g, tryptone 5g, FePO 4 0.1g, dilute seawater to 1L, pH 7.6—7.8; second: yeast extract 1g, tryptone 5g, FePO 4 0.1g, NaCl 34g, dilute to 1L with deionized water, pH 7.6—7.8). Inoculate the Edwardsiella tarda in the logarithmic growth phase into the above-mentioned medium at a ratio of 1:100, culture in a constant temperature incubator at 28°C, shake at 180rpm for 5h, centrifuge at 6000rpm at 4°C for 10min, collect the bact...

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Abstract

The invention relates to the technical field of precise inactivation of fish vaccines, in particular to a precise inactivation method for maintaining the immune protection of Edwardsiella antigen and an inactivation vaccine thereof. According to the influence of different inactivating agent concentrations, inactivating temperature and inactivating time on the inactivating effect, the present invention establishes an accurate inactivating model of formalin as the inactivating agent on Edwardsiella. This precise model determines the precise parameters that not only realize the effective inactivation of Edwardsiella, but also maintain its antigen immune protection. Select the exact combination of inactivating agent concentration, inactivating temperature and inactivating time within the range of 10min-48h to achieve precise inactivation of Edwardsiella, which is used to make Edwardsiella inactivated vaccines.

Description

technical field [0001] The invention relates to the technical field of precise inactivation of fish vaccines, in particular to a precise inactivation method for maintaining the immune protection of Edwardsiella antigen and an inactivation vaccine thereof. Background technique [0002] Edwardsiella spp. belongs to the Enterobacteriaceae, including three species of Edwardsiella tarda (E.tarda), Edwardsiella catfish (E.ictaluri) and Edwardsiella spp. (E.hoshinae), among which tarda Edwardsiella and Edwardsiella catfish are one of the main pathogenic bacteria in current aquaculture, causing diseases and causing huge losses to a variety of farmed fish worldwide. Edwardsiella tarda can infect fish such as: channel catfish (Ictaluruspunctatus), largemouth bass (Micropterus salmoides), eel (Anguillajapanica), mullet (Mugil cephalus), pterodon (Evynnisjaponica), tilapia (Tilapia nilotica) , Chinook salmon (Oncorhynchus tshawytscha), red snapper (Chrysophrysmajor), five-headed bream ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36A61K39/02A61P31/04
Inventor 谢国驷黄倢隋虎辰王秀华史成银许华杜迎彬张庆利李晨万晓媛
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI