Construction and Application of Radiosensitizing Fusion Polypeptide

Abstract

The invention discloses a construction method and applications of a radiotherapy sensitizing fusion polypeptide. According to the construction method, healthy nude mice are selected; nude-mouse transplanted tumor models are constructed via subcutaneous injection of human cervical carcinoma Hela cells as molding nude mice; the molding nude mice are divided into groups randomly, are injected with R9, R9-Ap(82-121) out-of-order peptides, R9-Ap(100-109) out-of-order peptides, R9-Ap(100-109), and R9-Ap(82-121 fusion polypeptide) respectively, and then are subjected to IR treatment and R9-Ap(100-109)+IR, R9-Ap(82-121)+IR treatment. In experiment processes, a vernier caliper is used for measuring the sizes of transplanted tumors, and the sizes are compared so as to evaluating radiotherapy sensitizing effect. The radiotherapy sensitizing fusion polypeptide is capable of realizing effective tumor cell targeting, increasing radiotherapy sensibility obviously at the same time, and possesses no toxic or side effect on normal tissue, is safe and reliable, and is excellent in effects.

Description

technical field [0001] The invention relates to the technical field of clinical medicine of tumors, in particular to the construction and application of radiotherapy-sensitizing fusion polypeptides. Background technique [0002] The incidence of malignant tumors is increasing year by year, which seriously threatens human health. At present, surgery, radiotherapy, and chemotherapy are still the main methods of tumor treatment. Among them, 65-70% of tumors require radiotherapy, and improving its efficacy is the key. However, since tumors are resistant to radiation, and normal tissues limit the increase of radiotherapy dose, the efficacy of radiotherapy is seriously affected; and when radiotherapy technology is used alone, the toxic and side effects are large, and the tumor is prone to recurrence, so the ideal curative effect cannot be achieved. The repair of DNA double-strand damage after radiation irradiation is an important reason for the resistance of tumor cells to radio...

AI Technical Summary

Problems solved by technology

The theoretical research on the use of some antineoplastic drugs as radiosensitizers has progressed rapidly, however, the toxic effect of these drugs on tissues is still a major factor limiting their use, and their mechanism of action is still not very clear

[0004] The application of radiotherapy sensitizers is a part of comprehensive treatment. At present, the clinical experimental research on radiotherapy sensitizers has achieved great results, but further research is needed to obtain clinically recognized low-toxic and effective sensitizers.

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