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Preparation method of exopolysaccharide from Cryptodinoflagellate and application of the exopolysaccharide

A technology of Cryptidinosa kouiii and exopolysaccharides, which is applied in the field of microbial fermentation, can solve the problems of anti-oxidation research and patents of undiscovered Cryptidium exopolysaccharides, and achieve the effect of eliminating oxidative damage to the body

Active Publication Date: 2019-09-24
PEKING UNIV +1
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Problems solved by technology

The applicant searched and found no research and patents on the application of Cryptidinosa exopolysaccharides in anti-oxidation

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  • Preparation method of exopolysaccharide from Cryptodinoflagellate and application of the exopolysaccharide
  • Preparation method of exopolysaccharide from Cryptodinoflagellate and application of the exopolysaccharide

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Embodiment

[0031] The preparation method of the exopolysaccharide of Cryptidium koushii comprises the following process steps:

[0032] A, Cultivate Cryptidium koirii: Inoculate the Cryptidium koirii strains into a sterile medium containing carbon source, nitrogen source, and nutrient factors to make a culture solution;

[0033] B. Obtaining a concentrated exopolysaccharide solution: centrifuging the culture medium, and filtering the supernatant to obtain a concentrated exopolysaccharide solution;

[0034] C. Obtaining exopolysaccharide: according to the ratio of absolute ethanol by volume: the concentrated exopolysaccharide solution obtained in step B = 2-4:1 ratio, in the concentrated exopolysaccharide solution obtained in step B, add no Water ethanol, exopolysaccharide was obtained after static precipitation;

[0035] D. Obtaining purified exopolysaccharide of Cryptidium kourii: dissolving the exopolysaccharide in step C into deionized water, and freeze-drying to obtain purified exop...

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Abstract

The invention belongs to microorganism fermentation, and particularly relates to a preparation method of crypthecodinium cohnii exopolysaccharides and application of the exopolysaccharides. The preparation method includes the process steps of crypthecodinium cohnii cultivation, exopolysaccharides concentrated solution obtaining and the like. The preparation method solves the technical problems that in the prior art, crypthecodinium cohnii exopolysaccharides are not effectively utilized and environment-friendly treatment of waste liquid generated by fermentation is complex. Crypthecodinium cohnii exopolysaccharides used by the method belong to the range of new resource food stated in China. Prepared crypthecodinium cohnii exopolysaccharides have good capacity for removing superoxide radicals, hydroxyl radicals and oxygen radicals, and good cell anti-oxidation vitality, have functional components corresponding to the anti-oxidation function, meet the requirements of corresponding specifications and standards of China, and can be effectively used for preparing antioxidant and making health care food with the anti-oxidation function.

Description

technical field [0001] The invention belongs to microbial fermentation, in particular to a method for preparing exopolysaccharides from Cryptodinium koushii and the application of the exopolysaccharides. Background technique [0002] Crypthecodinium cohnii belongs to the dinoflagellates, which often live in aquatic macroalgae and weakened seaweed, and are widely distributed in mangroves, oceans and other areas. Although the genes of Cryptidinium koii isolated from different regions are different, they all have good ability to produce docosahexaenoic acid (DHA). Crypthecodinium cohnii is the earliest source algae used for industrial fermentation to produce DHA. It has the advantages of short production cycle, easy large-scale cultivation, single unsaturated fatty acid composition, easy control of exogenous pollution, and not limited by raw material sources. , Has been included in my country's new resource food catalog. At present, the annual output of microalgae DHA is nearl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04A23L33/125C12R1/89
Inventor 陈峰刘宾姜悦
Owner PEKING UNIV
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