Preparation method of crypthecodinium cohnii exopolysaccharides and application of exopolysaccharides
A technology of Cryptodinium columbine and Cryptodinium columbine, which is applied in the field of preparation of exopolysaccharide of Crypthecodinium columbineum, can solve the problems such as research and patents on antioxidative aspects of exopolysaccharide of Cryptoninella columbine which have not been found, and achieves The effect of eliminating oxidative damage in the body
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[0031] The preparation method of the exopolysaccharide of Cryptidium koushii comprises the following process steps:
[0032] A, Cultivate Cryptidium koirii: Inoculate the Cryptidium koirii strains into a sterile medium containing carbon source, nitrogen source, and nutrient factors to make a culture solution;
[0033] B. Obtaining a concentrated exopolysaccharide solution: centrifuging the culture medium, and filtering the supernatant to obtain a concentrated exopolysaccharide solution;
[0034] C. Obtaining exopolysaccharide: according to the ratio of absolute ethanol by volume: the concentrated exopolysaccharide solution obtained in step B = 2-4:1 ratio, in the concentrated exopolysaccharide solution obtained in step B, add no Water ethanol, exopolysaccharide obtained after static precipitation;
[0035] D. Obtaining purified exopolysaccharide of Cryptidium kourii: dissolving the exopolysaccharide in step C into deionized water, and freeze-drying to obtain purified exopolys...
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