Preparation of two-color fluorescent gold bearing carbon dot and application of two-color fluorescent gold bearing carbon dot in visual inspection

A two-color fluorescence and carbon dot technology, applied in the field of applied chemistry, can solve the problems of complex and cumbersome preparation methods, and achieve the effects of obvious phenomena, fast visual detection, and high sensitivity

Active Publication Date: 2016-02-24
HUNAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, many preparation methods of gold-containing carbon dots have been studied, but most of these preparatio

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  • Preparation of two-color fluorescent gold bearing carbon dot and application of two-color fluorescent gold bearing carbon dot in visual inspection
  • Preparation of two-color fluorescent gold bearing carbon dot and application of two-color fluorescent gold bearing carbon dot in visual inspection
  • Preparation of two-color fluorescent gold bearing carbon dot and application of two-color fluorescent gold bearing carbon dot in visual inspection

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Embodiment 1

[0029] Preparation and compositional analysis of gold-containing carbon dots. Mix 5 mL, 20 mM freshly prepared chloroauric acid solution and 1.5 mL, 100 mM glutathione solution, and dilute it to 50 mL with ultrapure water. The solution was heated while stirring in an oil bath at 80°C for 2 hours. While still hot, it was transferred to an Erlenmeyer flask containing 2 g of glucose, and heated in a household microwave oven on high for 20 minutes. Dissolve the resulting tan product in 50 mL of ultrapure water again, centrifuge (16,000 rpm, 30 min), and dialyze the supernatant (molecular weight cut-off: 8000-14000) for 24 hours to obtain a pure gold-containing carbon dot solution . Measure the UV-Vis spectrogram and fluorescence spectrogram of gold-containing carbon dots with UV-Vis spectrophotometer and fluorescence spectrophotometer, such as figure 1 , figure 2 shown; and its TEM characterization, such as image 3 As shown, it can be seen that the size of the gold-containin...

Embodiment 2

[0031] Two-color imaging of gold-containing carbon dots in cells. Dissolve gold-containing carbon dots in water to prepare a 5 mg / mL aqueous solution, then take 1 mL of gold-containing carbon dots solution and 1 mL of breast cancer phosphate buffer solution, add 9 mL of cell culture medium, and incubate at 37 ° C for 4 hours, then use Laser confocal fluorescence microscope imaging, using 405nm excitation wavelength, to obtain two-color imaging of cells, such as Figure 5 , Figure 6 , Figure 7 , Figure 8 shown.

Embodiment 3

[0033] Visual inspection of PSA: Add 30 μL, 0.1 μM single-stranded DNA aptamer with thiol-modified chain end to 3 mL of the gold-containing carbon dot solution prepared in Example 1, react for 24 hours, dialyze (molecular weight cut-off 8000~14000), to remove unreacted DNA aptamers. Since DNA is negatively charged, the zeta potential of gold-containing carbon dots is significantly reduced, as shown in Figure 9 , Figure 10 shown; Figure 11The changes in the circular dichroism spectrum shown further illustrate the successful attachment of DNA to the surface of gold-containing carbon dots. Take 10 μL of different concentrations of PSA (1, 5, 10, 50, 100, 500, 1000 ng / mL) and add it to 90 μL of the gold carbon dot solution connected with the DNA aptamer, react at 37°C for 2 h, then add 100 μL of 5 mM TMB and 100 μL, 400 μM HO 2 o 2 After reacting for 15 minutes, a color gradient change with concentration changes appears, such as Figure 12 shown; and detect its UV-Vis abs...

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Abstract

The present invention discloses the preparation of a two-color fluorescent gold bearing carbon dot and application of the two-color fluorescent gold bearing carbon dot in visual inspection. The preparation of the gold bearing carbon dot is mainly as follows: a chloroauric acid solution and a glutathione solution are mixed and diluted with pure water; the mixed solution is heated while stirring for 1.5-2h in an oil bath at 80-100 DEG C, the mixed solution is hotly transferred into a conical flask containing glucose for heating for 20-25min at high fire in a domestic microwave oven; the resulting yellowish-brown product is dissolved with purified water, and centrifuged, the supernatant is dialyzed for 24hto obtain the product. Based on the nature of mimetic enzyme of peroxidase of the gold bearing carbon dot, the content of prostate cancer antigen in serum of patients with prostate cancer antigen can be detected by covering of two-color fluorescent gold bearing carbon dot catalytic surface sites by DNA aptamer and prostate cancer antigen specific effects, and fast visual detection of the prostate cancer antigen can be achieved. The method is simple, easy in operation, high in sensitivity, and has important significance in clinical medicine.

Description

technical field [0001] The invention belongs to the technical field of applied chemistry, and in particular relates to the preparation of a two-color fluorescent gold-containing carbon dot. Based on the catalytic performance of peroxidase, the gold-containing carbon dot realizes the visual detection of prostate cancer antigen. Background technique [0002] With the emergence of more and more nanomaterial preparation methods, gold-containing carbon dots have become an increasingly important class of materials. Due to the unique properties exhibited by single-component nanomaterials, gold-containing carbon dots have attracted more and more attention, which has enabled them to be used in a wide range of applications, such as catalysis, sensing, electronic applications, medical diagnosis, etc. So far, many preparation methods of gold-containing carbon dots have been studied, but most of these preparation methods are quite complicated and cumbersome, and they usually involve semi...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 黄昊文张凌阳刘兰芳周媛
Owner HUNAN UNIV OF SCI & TECH
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