Helicobacter pylori antibiotic resistance analysis kit and drug resistance detection method
An antibiotic resistance, Helicobacter pylori technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of few detection sites, high cost, low specificity, etc. achieve the effect of avoiding time-consuming
Active Publication Date: 2019-09-10
HUADONG HOSPITAL +1
View PDF3 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, the above inspection methods all have shortcomings such as few detection sites, low specificity, and low throughput, and cannot simultaneously perform drug resistance analysis
[0007] At present, the molecular biology technology for detecting antibiotic resistance of Helicobacter pylori still has the disadvantages of high cost and difficult clinical popularization. Identification and multi-site resistance analysis, so as to explore a new Helicobacter pylori detection and individualized Helicobacter pylori eradication model based on drug resistance analysis, so as to better guide the rational use of antibiotics in clinical practice and reduce the emergence of drug-resistant strains , reduce the financial burden on patients
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0075] 1. Materials
[0076] 1.1. Research object
[0077] H. pylori standard strain (ATCC 26695), two isolated H. pylori clinical strains.
[0078] 1.2. Materials and reagents
[0079]
[0080] 1.3. Instruments and equipment
[0081] CEQ TM 8000 Genetic Analysis System
[0082]
[0083] 2. Experimental method
[0084] 2.1. Nucleic acid extraction
[0085] Helicobacter pylori nucleic acid was extracted using Tiangen DNA Extraction Kit, and the method was carried out according to the instructions. The specific steps are as follows:
[0086] 1) Sample nucleic acid extraction preparation: equilibrate the reagents in the kit to room temperature, add 16ml of absolute ethanol to Buffer GD, mix well, and mark the added ethanol; add 68ml of absolute ethanol to Buffer PW, fully Mix well, and mark that ethanol has been added; mix thoroughly for later use; turn on the constant temperature bath, and set the temperature to 70°C.
[0087] 2) Arrange an appropriate...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| Sensitivity | aaaaa | aaaaa |
| Sensitivity | aaaaa | aaaaa |
Login to View More
Abstract
The invention provides a helicobacter pylori antibiotic drug resistance analysis kit and a method for detecting drug resistance of helicobacter pylori antibiotic through the kit. The kit comprises a primer. The primer is designed for drug resistance gene and identification gene 16S rRNA of helicobacter pylori antibiotic. On the basis of multiple gene analysis, all diagnosis genes are independent and clear, the rapid identification of helicobacter pylori and analysis of drug resistance of various antibiotics within the same reaction system can be achieved, and the defects that through a traditional cultivation method and drug sensitivity detection, consumed time is long, the positive rate is high and expenses are high are overcome.
Description
technical field [0001] The invention relates to a kit and a drug resistance detection method for detecting and analyzing the antibiotic resistance of Helicobacter pylori, in particular to an analysis kit and a detection method for the antibiotic resistance of Helicobacter pylori based on a multiple gene analysis system. Background technique [0002] Helicobacter Pylori (H. pylori) lives in the human stomach and is one of the most common bacterial pathogens. Half of the world's population has been infected by Helicobacter pylori. Helicobacter pylori infection may cause chronic gastritis and peptic ulcer, and in severe cases, it may develop into gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. [0003] With the widespread use of antibiotics, the drug-resistant strains of Helicobacter pylori are also increasing day by day. The resistance of Helicobacter pylori to antibiotics has become the main reason for the decline of its eradication rate, which not only...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/689
Inventor 保志军董方元黄一沁孔咪咪曾县平南丽吴勇余丁
Owner HUADONG HOSPITAL