A kit for simultaneous analysis of fluorescently labeled multiplexed amplification of 24 loci of human genome dna and its application
A technology of fluorescent labeling and compound amplification, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems of parent-child identification mutation close relative identification, database one child with multiple parents, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0078] Example 1 A kit for simultaneous analysis of fluorescently labeled multiplexed amplification of 24 loci of human genomic DNA, consisting of the following:
[0079] components
volume
Reaction Mix
10.0 μL
Genomic DNA
Xμl content is 0.125-5ng
Primers corresponding to the above 24 loci
5.0 μL
Hot start Taq enzyme (5U / μL)
0.5μL
wxya 2 o
Make up to 25.0 μL
[0080] The Reaction Mix mentioned in it is MgCl 2 7.5mM, Tris-HCl buffer 125mM, KCl 125mM, dNTPs7.5mM, BSA 2mg / mL.
[0081] The primers and primer concentrations corresponding to the 24 loci are:
[0082]
[0083]
[0084] The 5' end of at least one of the primers corresponding to each locus is labeled with a fluorescent dye.
[0085] The fluorescent dye marker is 6-FAM, HEX, TAMRA or ROX.
[0086] The 24 loci are grouped, and the following is one of the product combination methods: D16S539, D6S477, D15S659, D10S1248, D1S1656 and D21S2055 ...
Embodiment 2
[0100] Application of 24-locus fluorescence-labeled multiple amplification test system for triplet paternity identification
[0101] 1. Collect blood samples in paternity testing cases: the samples in this experiment are provided by XX paternity testing agency. DNA extraction Use the Chelex-100 method to extract genomic DNA from 3 whole blood samples: take 0.5-5 μl of whole blood and place it in a sterilized 1.5ml centrifuge tube, add 1ml sdH2O to the tube, shake for a few seconds; place at room temperature for 10 minutes Afterwards, shake for a few seconds, centrifuge at 12,000rpm for 3 minutes, discard the supernatant, keep enough supernatant to cover the precipitate, do not stir the precipitate; add 200μl 5% Chelex-100, shake for a few seconds; incubate at 56°C for 30 minutes, shake A few seconds; boiling water bath for 10 minutes, shake for a few seconds; centrifuge at 12,000rpm for 5 minutes, the supernatant is the extracted genomic DNA. Genomic DNA was extracted with re...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com