Maize mushroom production method and products
A corn mushroom and production method technology, applied in mushroom cultivation, botanical equipment and methods, horticulture, etc., can solve the impact of corn mushroom harvest quality on sales, processing, and consumption, affect the market acceptance of corn mushrooms, and have minimal utilization value and other problems, to achieve the effect of simple operation, meeting the needs of life, and high yield of mushrooms
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Embodiment 1
[0028] In this experiment, the isolation and cultivation of black powdery mildew was carried out in the headquarters of Jiangsu Academy of Agricultural Sciences, and the field inoculation was carried out in Liuhe Animal Science Base, including the following steps:
[0029] (1) Collection and cultivation of corn mushroom strains: corn smut was collected from Nanjing, Jiangsu, and a trace amount of mycelium heart juice was picked with aseptic picking needles under aseptic conditions, spread on the PDA medium, 28 Incubate at ℃ for 5 days, transfer all the grown colonies to a 2 ml sterile centrifuge tube, add an appropriate amount of sterile water to fully shake and suspend the bacterial cells, and finally add glycerol to the suspension to make the concentration of glycerol 15%. Store in -80°C refrigerator for long-term storage.
[0030] Preparation method of PDA medium: 200 pieces of potato-free, peeled and cut into small pieces, boiled in sterile water for 30 minutes, filtered, ...
Embodiment 2
[0036] In this experiment, the isolation and cultivation of black powdery mildew was carried out in the headquarters of Jiangsu Academy of Agricultural Sciences, and the field inoculation was carried out in Liuhe Animal Science Base, including the following steps:
[0037] (1) Collection and cultivation of corn mushroom strains: corn smut was collected from Nanjing, Jiangsu, and a trace amount of mycelium heart juice was picked with aseptic picking needles under aseptic conditions, spread on the PDA medium, 28 Incubate at ℃ for 5 days, transfer all the grown colonies to a 2 ml sterile centrifuge tube, add an appropriate amount of sterile water to fully shake and suspend the bacterial cells, and finally add glycerol to the suspension to make the concentration of glycerol 15%. Store in -80°C refrigerator for long-term storage.
[0038] (2) Preparation of inoculum: The inoculum was prepared by culturing smut before the corn bloomed. Take an appropriate amount of corn stigma liqu...
Embodiment 3
[0043] In this experiment, the isolation and cultivation of black powdery mildew was carried out in the headquarters of Jiangsu Academy of Agricultural Sciences, and the field inoculation was carried out in Liuhe Animal Science Base, including the following steps:
[0044](1) Collection and cultivation of corn mushroom strains: corn smut was collected from Nanjing, Jiangsu, and a trace amount of mycelium heart juice was picked with aseptic picking needles under aseptic conditions, spread on the PDA medium, 28 Incubate at ℃ for 5 days, transfer all the grown colonies to a 2 ml sterile centrifuge tube, add an appropriate amount of sterile water to fully shake and suspend the bacterial cells, and finally add glycerol to the suspension to make the concentration of glycerol 15%. Store in -80°C refrigerator for long-term storage.
[0045] (2) Preparation of inoculum: The inoculum was prepared by culturing smut before the corn bloomed. Take an appropriate amount of corn stigma liqui...
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