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Detection method for formaldehyde content in Spanish mackerel

A detection method and technology for formaldehyde content are applied in the field of detection of formaldehyde content in mackerel, can solve the problems of unfavorable detection and high cost of detection technology, and achieve the effects of convenient operation, low price and high detection accuracy

Active Publication Date: 2016-03-30
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on the high cost of the existing detection technology, which is not conducive to carrying out large-scale detection work, it is necessary to develop a quantitative detection method for formaldehyde content in Spanish mackerel that is easy to operate and low in cost.

Method used

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  • Detection method for formaldehyde content in Spanish mackerel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Materials and methods

[0035] 1.1 Materials

[0036] Spanish mackerel, bought at the farmers market

[0037] 1.2 Main instruments and reagents

[0038] 1.2.1 Main Instruments

[0039] Ultrasonic extractor; tissue masher; centrifuge; microburette; stoppered Erlenmeyer flask, volumetric flask.

[0040] 1.2.2 Reagents

[0041] Phenolphthalein indicator: weigh 1g of phenolphthalein, dissolve it in an appropriate amount of ethanol and dilute to 100ml;

[0042] The concentration of the substance is a sodium hydroxide solution of 0.1mol / L;

[0043] Mass percentage is 30% hydrogen peroxide;

[0044] The catalase (concentration is 1mg / ml) that the phosphate buffer solution configuration that uses pH is 7;

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Abstract

The invention relates to a detection method for formaldehyde content in Spanish mackerel, belonging to the field of food safety of aquatic products. The detection method comprises the following steps: first preprocessing the Spanish mackerel, then extracting formaldehyde in the preprocessed Spanish mackerel with an ultrasonic extraction method to obtain crude extract, purifying the crude extract to obtain purified formaldehyde extract, adding hydrogen dioxide solution in an oxidizing reaction step so that the formaldehyde in the purified formaldehyde extract is oxidized into formic acid, titrating the formic acid with sodium hydroxide to obtain the content of the formic acid, setting a blank test and calculating the formaldehyde content in the Spanish mackerel according to the result of the blank test and the content of the formic acid, wherein phenolphthalein is taken as an indicator in the titration step. According to the detection method, the formaldehyde in the Spanish mackerel can be effectively extracted and detected, and the detection cost is low.

Description

technical field [0001] The invention belongs to the field of food safety of aquatic products, and in particular relates to a method for detecting formaldehyde content in Spanish mackerel. Background technique [0002] In recent years, the food safety of aquatic products has attracted more and more people's attention, especially the content of formaldehyde in aquatic products is one of the main targets of people's attention. Main sources of formaldehyde: First, artificial addition. Aquatic products are easily spoiled during the logistics and storage process. In order to prolong the preservation and sales time and improve the appearance of aquatic products, some unscrupulous vendors often soak aquatic products with formaldehyde, which directly endangers consumption. The second is that aquatic products are naturally produced. During the storage process, aquatic products can produce formaldehyde by themselves under the action of enzymes and microorganisms. The formaldehyde produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 励建荣张丽华李灿婴孙协军马春颖
Owner BOHAI UNIV
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