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Applications of chrysophanol or derivative thereof and oncolytic virus in preparation of antitumor drugs

An oncolytic virus and chrysophanol technology are applied in the field of biomedicine to achieve the effects of increasing anti-tumor effect, enhancing inhibitory effect and improving therapeutic effectiveness

Active Publication Date: 2016-04-06
GUANGZHOU VIROTECH PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the M1 antitumor synergist

Method used

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  • Applications of chrysophanol or derivative thereof and oncolytic virus in preparation of antitumor drugs
  • Applications of chrysophanol or derivative thereof and oncolytic virus in preparation of antitumor drugs
  • Applications of chrysophanol or derivative thereof and oncolytic virus in preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Chrysophanol and M1 virus significantly increase the morphological lesions of human hepatocellular carcinoma lines

[0041] Material:

[0042] Human hepatocellular carcinoma Hep3B, M1 virus, high glucose DMEM medium, inverted phase contrast microscope.

[0043] method:

[0044]a) Cell culture: Human hepatocellular carcinoma Hep3B was grown in DMEM complete medium containing 10% FBS, 100 U / ml penicillin and 0.1 mg / ml streptomycin; all cell lines were placed in 5% CO 2 , cultured and subcultured in a closed incubator with a constant temperature of 37°C (95% relative humidity), and observed the growth with an inverted microscope. The cells were subcultured every 2-3 days, and the cells in the logarithmic growth phase were taken for formal experiments.

[0045] b) Cell treatment and morphological observation: cells in the logarithmic growth phase were selected, and DMEM complete culture medium (containing 10% fetal bovine serum, 1% double antibody) was used to ...

Embodiment 2

[0048] Example 2 Combined treatment of chrysophanol and M1 virus significantly reduces the survival rate of human hepatocellular carcinoma strains

[0049] Material:

[0050] Human hepatocellular carcinoma Hep3B, M1 virus, high glucose DMEM medium, automatic enzyme-linked detection microplate reader.

[0051] method:

[0052] a) Cell inoculation, administration treatment: select logarithmic growth phase cells, DMEM complete culture medium (containing 10% fetal calf serum, 1% double antibody) to make cell suspension, with 4 × 10 3 The density per well was seeded in a 96-well culture plate. After 12 hours, the cells were completely attached to the wall. The experiments were divided into control group, chrysophanol alone group, M1 infection group and chrysophanol / M1 combined group. The doses used were: M1 virus (MOI=0.001) infected cells; different dose gradients were set for chrysophanol.

[0053] b) MTT reacts with intracellular succinate dehydrogenase: when cultured for 72...

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Abstract

The invention belongs to the field of biomedicines, and relates to applications of chrysophanol or a derivative thereof and oncolytic virus in preparation of antitumor drugs. The invention finds that chrysophanol or the derivative thereof can be used for preparing oncolytic virus antitumor synergistic agents for the first time. Meanwhile, the invention also relates to a pharmaceutical composition containing chrysophanol and oncolytic virus, a medicine suit containing chrysophanol and oncolytic virus, and applications of chrysophanol and oncolytic virus in treatment of tumors, particularly tumors insensitive to the oncolytic virus.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of the combination of chrysophanol and oncolytic virus in the preparation of antitumor drugs. Background technique [0002] Oncolytic virus (oncolytic virus) is a kind of replication-competent virus that targets and infects and kills tumor cells without destroying normal cells. Oncolytic virus therapy (oncolytic virus therapy) is an innovative tumor-targeted treatment strategy, which uses natural or genetically engineered viruses to selectively infect tumor cells and replicate in tumor cells to achieve targeted dissolution and killing The effect of tumor cells, but harmless to normal cells. [0003] M1 virus (AlphavirusM1) belongs to the genus Alphavirus (Alphavirus), and its application in the preparation of antitumor drugs has been recorded in Chinese invention patent application 201410425510.3. The M1 virus can selectively cause the death of tumor cells without affect...

Claims

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Application Information

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IPC IPC(8): A61K35/768A61K35/766A61K35/763A61K35/761A61P35/00A61K31/122
Inventor 颜光美张海鹏高光坪程世源毛子旭郑明豪林穗珍龚守芳胡骏朱文博银巍
Owner GUANGZHOU VIROTECH PHARMA
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