Supercharge Your Innovation With Domain-Expert AI Agents!

Human bocavirus LAMP (loop-mediated isothermal amplification) detection kit

A technology of human Boca virus and Boca virus, which is applied in the field of molecular biology detection of viruses, can solve the problems of expensive PCR instruments, cumbersome operation, and guiding functions, and achieve the effect of simple result identification and efficient amplification

Active Publication Date: 2016-04-06
首都儿科研究所
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although detection methods based on PCR technology have been established, PCR requires expensive instruments and is cumbersome to operate
In addition, the existing detection methods cannot achieve rapid diagnosis in a specific single specimen (for example, only blood samples, or only excreta) in terms of specificity or detection time limit, which makes the existing detection methods require and rely on samples High reliability, unable to play a guiding role in the clinical very early or early effective treatment of human bocavirus infection and further limit their large-scale clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human bocavirus LAMP (loop-mediated isothermal amplification) detection kit
  • Human bocavirus LAMP (loop-mediated isothermal amplification) detection kit
  • Human bocavirus LAMP (loop-mediated isothermal amplification) detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0058] 1. A primer set for amplifying human bocavirus gene sequence, comprising a combination of sequences shown in SEQNOID: 2-7.

[0059] 2. The primer set according to embodiment 1, characterized in that the human bocavirus gene sequence is the sequence shown in SEQNOID:1.

[0060] 3. A kit for rapid detection of human bocavirus, characterized in that the kit comprises the primer set according to embodiments 1 and 2.

[0061] 4. The kit according to embodiment 3, characterized in that the kit further comprises dNTP and BstDNA polymerase.

[0062] 5. Use of the primer set according to embodiment 1 or 2 in the preparation of a kit for detecting human bocavirus.

[0063] 6. A method for detecting human bocavirus, the method comprising the following steps:

[0064] 1) providing a sample to be tested obtained from the subject, and extracting the DNA of the sample to be tested;

[0065] 2) using the DNA of the sample to be tested as a template, and using primers having sequence...

Embodiment 1

[0077] Embodiment 1, be used for the primer design that human bocavirus is carried out LAMP detection

[0078] The human Bocavirus N gene sequence (SEQNOID: 1) was retrieved from the American Gene Database, and homology analysis was performed by BLAST software to obtain the specific conserved target sequence of the human Bocavirus, and then according to the conserved target DNA sequence, the software was used to PrimerdesignV4 designed primers for LAMP detection of human bocavirus, designed 3 sets of primers (HBoV13-1, HBoV13-2, HBoV13-3), after experimental comparison, finally selected the primer combination HBoV13 consisting of 6 primers -1, the primer sequences are shown in Table 1.

[0079] Table 1 is used for the primer HBoV13-1 that human bocavirus is carried out LAMP detection

[0080]

Embodiment 2

[0081] Embodiment 2, the establishment of the LAMP detection method of human bocavirus of the present invention

[0082] The six primer combinations HBoV13-1 obtained in Example 1 for carrying out LAMP detection to human Boca virus were used to carry out LAMP detection to human Boca virus strains (from the Capital Institute of Pediatrics), in order to obtain the best reaction system and reaction conditions, At the same time, the other two sets of primers (HBoV13-2, HBoV13-3) were used as controls, and the specific method was as follows:

[0083] 1. Determination of the best response system

[0084] Under the same reaction conditions (for example, keep the temperature at 63° C. for 90 minutes), the reaction system includes the following substances, and the following is the determined optimal reaction system:

[0085] 1) With human bocavirus genomic DNA (using DNAzol nucleic acid extraction reagent to extract the nucleic acid in the sample to be tested) as a template, under the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a primer group used for amplifying a gene sequence of human bocavirus and a kit containing the primer group for rapidly detecting the human bocavirus. The primer group disclosed by the invention contains the combination of sequences as shown in SEQ NO ID: 2-7. The invention also discloses a method for detecting the human bocavirus by utilizing the primer group disclosed by the invention. The kit and / or the detection method can rapidly, conveniently, efficiently, highly specifically and highly sensitively detect the human bocavirus under an isothermal condition, do not need complex instruments, and can be used for screening of children's infectious diseases and detecting the human bocavirus.

Description

technical field [0001] The invention belongs to the field of molecular biology detection of viruses, and in particular relates to a primer set for detecting human bocavirus, a detection kit comprising the primer set and a corresponding detection method. More specifically, the present invention relates to a special primer set for detecting human bocavirus by loop-mediated isothermal amplification technique (LAMP), a detection kit comprising the special primer set and a corresponding detection method. Background technique [0002] Human bocavirus (Humanbocavirus, HBoV) is a new virus discovered in the nasopharyngeal secretions of children with acute respiratory infection by Swedish scientist Allander et al. in 2005. Viruses (Bocavirus). The family Parvoviridae includes the subfamilies Parvoviridae and Densoviridae. The parvovirinae are vertebrate viruses, and the densovirinae are insect viruses. In addition to B19 parvovirus and human parvovirus 4 (Humanparvovirus, PARV4), ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/6844C12Q1/70
Inventor 赵林清钱渊朱汝南
Owner 首都儿科研究所
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com