A thermal shock gasification electrospray ionization source and mass spectrometry system

A technology of electrospray mass spectrometry and thermal shock, which is applied in the direction of mass spectrometer, ion source/gun, particle separator tube parts, etc., and can solve the problems of chromatographic system coupling, low efficiency of applying heat energy to samples, and easy decomposition of sample molecules when heated etc. to achieve high ionization efficiency, improve application efficiency, and shorten heating time

Active Publication Date: 2017-11-24
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current method of thermal desorption is inefficient in applying thermal energy to the sample, which leads to the easy decomposition of sample molecules when heated. It is only suitable for the analysis of small molecules that are thermally stable and volatile, and it is difficult to combine with a chromatographic system, thus limiting this method. application of technology

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  • A thermal shock gasification electrospray ionization source and mass spectrometry system
  • A thermal shock gasification electrospray ionization source and mass spectrometry system
  • A thermal shock gasification electrospray ionization source and mass spectrometry system

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Embodiment 1

[0038] figure 1 As a specific embodiment of the thermal shock gasification electrospray ionization mass spectrometry system of the present invention, the mass spectrometry system includes two parts, that is, a mass spectrometer part and an ionization source part. Mass spectrometers are of a great variety and are widely used. The ionization source provided by the invention can be connected with any mass spectrometer with an atmospheric pressure interface, such as ion trap mass spectrometry, quadrupole mass spectrometry, time-of-flight mass spectrometry, Fourier transform ion cyclotron resonance mass spectrometry, Magnetic mass spectrometry, orbitrap mass spectrometry, etc. The mass spectrometer of the present invention includes a mass spectrometer inlet 4, through which the ions generated by the ionization source can be introduced into the mass analyzer for mass spectrometry analysis. The ionization source part is the thermal shock gasification electrospray ionization source ...

Embodiment 2

[0050] The thermal shock gasification electrospray ionization mass spectrometry system of the present invention is used to analyze thermally unstable compounds, and mesaconitine is used as the detection object. Add 100 μL of 10 mg / L aconitine methanol solution dropwise to the bottom of the unheated vaporization chamber, and at the same time turn on the electrospray, record the mass spectrometry data, and heat the bottom of the vaporization chamber to 320°C within 2 minutes to gradually vaporize it , but in addition to the background signal of the electrospray solvent in the collected mass spectrometry data (the spectrum is not listed), there is no signal related to mesoaconitine, indicating that mesoaconitine is completely decomposed during the heating process. Using the thermal shock gasification electrospray ionization method described in the present invention, first heat the bottom of the gasification chamber to 320°C, and then apply the methanol solution of aconitine to the...

Embodiment 3

[0052] The thermal shock gasification electrospray ionization mass spectrometry system of the present invention is used to analyze biomacromolecules, and horse heart cytochrome C (cytochrome c) is used as the detection object. The electrospray solvent is a mixed solution of methanol / water (1:1) containing 0.1% formic acid, and 100 μL of 0.31 mg / ml cytochrome C aqueous solution is added dropwise to the bottom of the unheated vaporization chamber. At the same time, the electrospray is turned on, and the mass spectrometry data is recorded. Afterwards, the vaporization chamber was heated to 400°C within 2 minutes to gradually vaporize it. However, the collected mass spectrometry data did not show any correlation with cytochrome C except for the background signal of the electrospray solvent (the spectrogram is not listed). The signal indicated that cytochrome C was denatured during the heating process and did not enter the mass spectrometry system.

[0053] Using the thermal shock ...

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Abstract

The invention discloses a thermal shock gasification electrospray ionization source and a mass spectrometry analysis system, and belongs to the technical field of normal pressure mass spectrometry ionization. The mass spectrometer ionization source of the present invention comprises an electrospray generating device, a heating device and a sampling device, wherein the heating device comprises a gasification chamber and a heater providing heat for the gasification chamber, and the sample tube nozzle of the sampling device faces the gasification chamber Bottom setting. The outlet end of the spray capillary of the electrospray generating device is opposite to the inlet of the mass spectrometer at a certain distance, and the gasification chamber and the sample nozzle are located below the distance. The ionization source provided by the invention vaporizes the sample in a thermal shock manner, and improves the heating efficiency of the sample by shortening the heating time. Due to the short heating time of the sample, the heat energy is not converted into the internal energy of the sample molecules, and is only provided to the sample to overcome the intermolecular force, and the sample molecules will not be decomposed by heat, so that thermally unstable, non-volatile compounds and biological samples can be detected, which solves the problem Difficulties of traditional thermal desorption technology.

Description

technical field [0001] The invention relates to a thermal shock gasification electrospray ionization source and a mass spectrometry analysis system, belonging to the technical field of atmospheric mass spectrometry ionization. Background technique [0002] The ratio (mass-to-charge ratio) of the charge on the analyte molecule to its mass (mass-to-charge ratio) during the analysis process can be ascertained by mass spectrometry (Mass spectrometry, MS) analysis technology, so that the molecular weight information of the molecule or its fragments can be obtained. As an important analytical technique, mass spectrometry has the advantages of high sensitivity, high throughput, and good quantitative linearity. The first mass spectrometer was made in 1919. Since then, mass spectrometry technology has developed rapidly in the field of analytical chemistry, and has become a major instrument in chemistry, medicine, materials, biology, medicine, environment, energy, food, criminal foren...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01J49/10H01J49/26
CPCH01J49/10H01J49/26
Inventor 许庆轩
Owner HEILONGJIANG UNIV
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