A kind of efficient extraction method of hemolymph of bivalve mollusk

Abstract

The invention relates to a method for efficiently extracting hemolymph of bivalve. The method includes the following steps that a, seawater on the surface of the bivalve is completely absorbed through cotton balls, the surface of the bivalve is wiped with ethyl alcohol to be sterilized, and seawater on the surface of the bivalve is completely sucked up through cotton balls; b, the bivalve which is completely wiped is placed in a groove of an extracting device, the adductor muscle of the bivalve is ripped open, a shell is open, and two shells are flatly open and erected in the groove; c, after pelecypod of the bivalve and the portion below a water pipe are cushioned with cotton balls, an outer sleeve membrane above a pericardial cavity is slightly shifted away through tweezers, and the pericardial cavity is completely exposed; d, a gun head of a liquid moving device is cut, meanwhile, the pelecypod of the bivalve is stimulated, and hemolymph is fast sucked with the liquid moving device when the heart beats and the pericardial cavity swells. The method has the advantages that the method is easy to operate, the total amount of the extracted hemolymph of the bivalve can be effectively increased, the purity of the hemolymph is improved, and the operating environment can be kept clean in the operating process.

Description

【Technical field】 [0001] The invention relates to the technical field of bivalve hemolymph extraction, in particular to an efficient bivalve hemolymph extraction method. 【Background technique】 [0002] Today is the age of the ocean, and the aquaculture industry is developing rapidly. As an important economic aquatic animal, bivalve mollusks have always been the key factor for high yield in production practice, and have been paid close attention by researchers. As an important immune tissue of shellfish, hemolymph has extremely high research value. [0003] Because bivalve mollusks have an open-tube circulatory system, the collection of their hemolymph has always been difficult. At present, researchers mostly use syringes and needles. However, the traditional method of taking blood with syringes and needles has too thin needles, which are easy to block during suction, which often results in insufficient suction, and it is easy to draw impure body fluids. [0004] Based on ...

AI Technical Summary

Problems solved by technology

At present, researchers mostly use syringes and needles. However, the traditional method of using syringes and needles to draw blood has too thin needles and is easy to clog when drawing, which often leads to insufficient suction and impure body fluids.

[0004] Based on the above reasons, in the process of extracting hemolymph, the low purity and small amount have always been a problem for researchers.

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