Preparation method and detection method of nano-immunosensor used for rapidly detecting benzo(a)pyrene

An immunosensor and benzo-based technology, which is applied in the field of nano-immunosensor preparation, can solve the problems of poor resolution of results, cumbersome operation, long time consumption, etc., and achieve the effects of simple preparation, improved detection efficiency, and simplified sample pretreatment steps.

Inactive Publication Date: 2016-04-13
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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  • Application Information

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Problems solved by technology

The reported detection methods of benzo(a)pyrene include liquid chromatography, gas chromatography-mass spectrometry, thin-layer chromatography, etc., but the detection methods such as chromatography and chromatography are cumbersome, time-consuming, and poor in resolution. and other shortcomings
Therefore, many researchers are currently working on developing a more rapid and accurate detection method for benzo(a)pyrene to improve the detection efficiency of benzo(a)pyrene in food, while avoiding the phenomenon of false negative or false positive

Method used

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  • Preparation method and detection method of nano-immunosensor used for rapidly detecting benzo(a)pyrene
  • Preparation method and detection method of nano-immunosensor used for rapidly detecting benzo(a)pyrene
  • Preparation method and detection method of nano-immunosensor used for rapidly detecting benzo(a)pyrene

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Embodiment 1

[0039] Detection of benzo(a)pyrene in meat products I by nano-immunosensor

[0040] 1. A preparation method for a nano-immunosensor for rapid detection of benzo(a)pyrene

[0041] a. Electrode pretreatment

[0042] Polish the glassy carbon electrode with alumina polishing powder with a particle size of 0.05 μm until the surface of the electrode becomes a mirror surface, then rinse it with distilled water, and then place it in nitric acid, acetone, and deionized water with a volume fraction of 50% for ultrasonic cleaning for 3 minutes. Blow dry with nitrogen, set aside;

[0043] b. Preparation of polyamide-amine-PDDA functionalized graphene composite

[0044] Accurately weigh 0.2 g of graphene and add it to 10 mL of polydiallyldimethylammonium chloride aqueous solution (PDDA, 0.25% by volume), ultrasonicate for 30 min, wash with water three times to remove excess graphene, and obtain PDDA functionalized Add 0.01g of PDDA-functionalized graphene to 10mL N,N-dimethylformamide t...

Embodiment 2

[0058] Detection of benzo(a)pyrene in meat products II by nano-immunosensor

[0059] 1. A preparation method for a nano-immunosensor for rapid detection of benzo(a)pyrene

[0060] a. Electrode pretreatment

[0061] Same as Example 1

[0062] b. Preparation of polyamide-amine-graphene composite

[0063] Same as Example 1

[0064] c. Preparation of gold nanoparticles

[0065] Same as Example 1

[0066] d. Preparation of nano-immunosensors

[0067] Apply the glassy carbon electrode obtained in step a to a three-electrode system, that is, use the glassy carbon electrode as the working electrode, the Ag / AgCl electrode as the reference electrode, and the platinum wire electrode as the auxiliary electrode, in the potential range of -0.2V to +0.6V , using LK98BⅡ micro-electrochemical analysis system with 50mV·s -1The scanning speed is 10 cycles of cyclic voltammetry scanning in the newly prepared 0.05mol / L sulfuric acid solution, and the surface of the glassy carbon electrode i...

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Abstract

The invention belongs to the technical field of rapid detection of food safety, and particularly relates to a preparation method and a detection method of a nano-immunosensor used for rapidly detecting benzo(a)pyrene. The preparation method includes the steps that a glassy carbon electrode serves as a basic electrode, a polyamide-amne dendrimer is combined with poly(diallyldimethylammonium chloride) functionalized graphene for modifying the glassy carbon electrode, then a nano-gold solution is dispensed, nano-gold is immobilized to the modified electrode under the electrostatic adsorption effect, and then a polycyclic aromatic hydrocarbon antibody is immobilized through glutaraldehyde coupling to prepare the nano-immunosensor. The nano-immunosensor is used for detecting benzo(a)pyrene in food; a result obtained through the detection method is consistent with that obtained through a national standard method, but the detection method for benzo(a)pyrene is high in sensitivity and accuracy, short in detection time, simple and convenient and fast to implement and has broad application prospects.

Description

technical field [0001] The invention relates to a preparation method of a nano-immunosensor for rapid detection of benzo(a)pyrene, and a method for detecting benzo(a)pyrene in meat products by using the nano-immunosensor. Background technique [0002] Benzo(a)pyrene is a polycyclic aromatic hydrocarbon composed of 4 benzene rings. It is a colorless to pale yellow needle-like crystal at room temperature and is stable in nature. In the process of frying, grilling, and frying animal meat products, the food will produce benzo(a)pyrene, and human and animals will cause tumors and cancers after ingesting meat products with benzo(a)pyrene exceeding the limit. Health poses a potential threat. At present, the disease caused by food contamination is one of the food safety issues that have attracted widespread attention. Many countries have made benzo(a)pyrene one of the important contents of the monitoring of food harmful substances. For example, Germany restricts benzo(a)pyrene in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 李书国王瑞鑫冯亚净张云焕
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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